Deletion polymorphism of SIGLEC14 and its functional implications

Masahiro Yamanaka, Yukinari Kato, Takashi Angata, Hisashi Narimatsu

Research output: Contribution to journalArticlepeer-review

73 Citations (Scopus)

Abstract

Human Siglec-14, a member of the Siglec family of sialic acid-binding lectins, shows extensive sequence similarity to human Siglec-5. To analyze respective expression patterns of Siglec-14 and Siglec-5, we developed specific antibodies against each of them. We found that the former was expressed on granulocytes and monocytes, while the latter was on granulocytes and B-cells. Surprisingly, some individuals lacked the expression of Siglec-14, while they all expressed Siglec-5. We found that a fusion between SIGLEC14 and SIGLEC5 genes, resulting in the functional deletion of SIGLEC14, underlies this phenotype. The presence of the "SIGLEC14 null" allele in all human populations we tested implies an ancient origin, while its allelic frequency is higher in Asians compared with Africans and Europeans. The forced expression of Siglec-14 in a monocytic cell line-enhanced TNF-α secretion elicited by lipopolysaccharide. These results imply that Siglec-14 may play some role in bacterial infection.

Original languageEnglish
Pages (from-to)841-846
Number of pages6
JournalGlycobiology
Volume19
Issue number8
DOIs
Publication statusPublished - 2009
Externally publishedYes

Keywords

  • DAP12
  • Leukocytes
  • Polymorphism
  • Siglec
  • TNF-α

ASJC Scopus subject areas

  • Biochemistry

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