TY - JOUR
T1 - Delimitation of cohesive ends site (cos) of Streptomyces temperate bacteriophage R4
AU - Mitsui, Hisayuki
AU - Takahashi, Hideo
N1 - Funding Information:
This work was supported research from the Ministry of Japan and from the Institute (RIKEN).
PY - 1992/8/14
Y1 - 1992/8/14
N2 - The cohesive ends site (cos) of actinophage R4 was delimitated by assaying the in vivo packaging activity of cosmid derivatives in Streptomyces lividans. A region of 66 by from -30 to +36 from the center of cohesive ends was required for the basal level of packaging activity. Two additional regions outside the basal sequences from -39 to -31 and from +37 to +97 were necessary for the high level of activity, defined as the accessory sequences. Direct- or inverted-repeat sequences were found within the delimitated region, which might be involved in the recognition by the terminase of actinophage R4.
AB - The cohesive ends site (cos) of actinophage R4 was delimitated by assaying the in vivo packaging activity of cosmid derivatives in Streptomyces lividans. A region of 66 by from -30 to +36 from the center of cohesive ends was required for the basal level of packaging activity. Two additional regions outside the basal sequences from -39 to -31 and from +37 to +97 were necessary for the high level of activity, defined as the accessory sequences. Direct- or inverted-repeat sequences were found within the delimitated region, which might be involved in the recognition by the terminase of actinophage R4.
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U2 - 10.1016/S0006-291X(05)81599-2
DO - 10.1016/S0006-291X(05)81599-2
M3 - Article
C2 - 1514959
AN - SCOPUS:0027005023
SN - 0006-291X
VL - 186
SP - 1663
EP - 1668
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -