Delimitation of cohesive ends site (cos) of Streptomyces temperate bacteriophage R4

Hisayuki Mitsui, Hideo Takahashi

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


The cohesive ends site (cos) of actinophage R4 was delimitated by assaying the in vivo packaging activity of cosmid derivatives in Streptomyces lividans. A region of 66 by from -30 to +36 from the center of cohesive ends was required for the basal level of packaging activity. Two additional regions outside the basal sequences from -39 to -31 and from +37 to +97 were necessary for the high level of activity, defined as the accessory sequences. Direct- or inverted-repeat sequences were found within the delimitated region, which might be involved in the recognition by the terminase of actinophage R4.

Original languageEnglish
Pages (from-to)1663-1668
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number3
Publication statusPublished - 1992 Aug 14


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