TY - JOUR
T1 - Dentin regeneration by dental pulp stem cell therapy with recombinant human bone morphogenetic protein 2
AU - Iohara, K.
AU - Nakashima, M.
AU - Ito, M.
AU - Ishikawa, M.
AU - Nakasima, A.
AU - Akamine, A.
N1 - Funding Information:
We are grateful to Yamanouchi Pharmaceutical Co., Ltd. for providing recombinant human BMP2. This work was supported by a Grant-in-Aid for Scientific Research (#13470403) from the Ministry of Education, Science, Sports and Culture, Japan.
PY - 2004/8
Y1 - 2004/8
N2 - Regenerative medicine is based on stem cells, signals, and scaffolds. Dental pulp tissue has the potential to regenerate dentin in response to noxious stimuli, such as caries. The progenitor/stem cells are responsible for this regeneration. Thus, stem cell therapy has considerable promise in dentin regeneration. Culture of porcine pulp cells, as a three-dimensional pellet, promoted odontoblast differentiation compared with monolayers. The expression of dentin sialophosphoprotein (Dspp) and enamelysin/matrix metalloproteinase 20 (MMP20) mRNA confirmed the differentiation of pulp cells into odontoblasts and was stimulated by the morphogenetic signal, bone morphogenetic protein 2 (BMP2). Based on the in vitro experiments, an in vivo evaluation of pulp progenitor/stem cells in the dog was performed. The autogenous transplantation of the BMP2-treated pellet culture onto the amputated pulp stimulated reparative dentin formation. In conclusion, BMP2 can direct pulp progenitor/stem cell differentiation into odontoblasts and result in dentin formation. Abbreviations: BMP2, bone morphogenetic protein 2; Dspp, dentin sialophosphoprotein; Dmp1, dentin matrix protein 1; ALPase, alkaline phosphatase; MMP20, matrix metalloproteinase 20; Phex, phosphate-regulating gene with homologies to endopeptidases on X-chromosome.
AB - Regenerative medicine is based on stem cells, signals, and scaffolds. Dental pulp tissue has the potential to regenerate dentin in response to noxious stimuli, such as caries. The progenitor/stem cells are responsible for this regeneration. Thus, stem cell therapy has considerable promise in dentin regeneration. Culture of porcine pulp cells, as a three-dimensional pellet, promoted odontoblast differentiation compared with monolayers. The expression of dentin sialophosphoprotein (Dspp) and enamelysin/matrix metalloproteinase 20 (MMP20) mRNA confirmed the differentiation of pulp cells into odontoblasts and was stimulated by the morphogenetic signal, bone morphogenetic protein 2 (BMP2). Based on the in vitro experiments, an in vivo evaluation of pulp progenitor/stem cells in the dog was performed. The autogenous transplantation of the BMP2-treated pellet culture onto the amputated pulp stimulated reparative dentin formation. In conclusion, BMP2 can direct pulp progenitor/stem cell differentiation into odontoblasts and result in dentin formation. Abbreviations: BMP2, bone morphogenetic protein 2; Dspp, dentin sialophosphoprotein; Dmp1, dentin matrix protein 1; ALPase, alkaline phosphatase; MMP20, matrix metalloproteinase 20; Phex, phosphate-regulating gene with homologies to endopeptidases on X-chromosome.
KW - BMP2
KW - Dental pulp-capping
KW - Dentin regeneration
KW - Pellet culture
KW - Stem cell therapy
UR - http://www.scopus.com/inward/record.url?scp=4444361765&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=4444361765&partnerID=8YFLogxK
U2 - 10.1177/154405910408300802
DO - 10.1177/154405910408300802
M3 - Article
C2 - 15271965
AN - SCOPUS:4444361765
SN - 0022-0345
VL - 83
SP - 590
EP - 595
JO - Journal of Dental Research
JF - Journal of Dental Research
IS - 8
ER -