Dentin regeneration by dental pulp stem cell therapy with recombinant human bone morphogenetic protein 2

K. Iohara, M. Nakashima, M. Ito, M. Ishikawa, A. Nakasima, A. Akamine

Research output: Contribution to journalArticlepeer-review

319 Citations (Scopus)


Regenerative medicine is based on stem cells, signals, and scaffolds. Dental pulp tissue has the potential to regenerate dentin in response to noxious stimuli, such as caries. The progenitor/stem cells are responsible for this regeneration. Thus, stem cell therapy has considerable promise in dentin regeneration. Culture of porcine pulp cells, as a three-dimensional pellet, promoted odontoblast differentiation compared with monolayers. The expression of dentin sialophosphoprotein (Dspp) and enamelysin/matrix metalloproteinase 20 (MMP20) mRNA confirmed the differentiation of pulp cells into odontoblasts and was stimulated by the morphogenetic signal, bone morphogenetic protein 2 (BMP2). Based on the in vitro experiments, an in vivo evaluation of pulp progenitor/stem cells in the dog was performed. The autogenous transplantation of the BMP2-treated pellet culture onto the amputated pulp stimulated reparative dentin formation. In conclusion, BMP2 can direct pulp progenitor/stem cell differentiation into odontoblasts and result in dentin formation. Abbreviations: BMP2, bone morphogenetic protein 2; Dspp, dentin sialophosphoprotein; Dmp1, dentin matrix protein 1; ALPase, alkaline phosphatase; MMP20, matrix metalloproteinase 20; Phex, phosphate-regulating gene with homologies to endopeptidases on X-chromosome.

Original languageEnglish
Pages (from-to)590-595
Number of pages6
JournalJournal of dental research
Issue number8
Publication statusPublished - 2004 Aug
Externally publishedYes


  • BMP2
  • Dental pulp-capping
  • Dentin regeneration
  • Pellet culture
  • Stem cell therapy

ASJC Scopus subject areas

  • Dentistry(all)


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