TY - JOUR
T1 - Detection of an immunogenic HERV-E envelope with selective expression in clear cell kidney cancer
AU - Cherkasova, Elena
AU - Scrivani, Claire
AU - Doh, Susan
AU - Weisman, Quinn
AU - Takahashi, Yoshiyuki
AU - Harashima, Nanae
AU - Yokoyama, Hisayuki
AU - Srinivasan, Ramaprasad
AU - Linehan, W. Marston
AU - Lerman, Michael I.
AU - Childs, Richard W.
N1 - Publisher Copyright:
© 2016 American Association for Cancer Research.
PY - 2016/4/15
Y1 - 2016/4/15
N2 - VHL-deficient clear cell renal cell carcinomas (ccRCC), the most common form of kidney cancer, express transcripts derived from the novelhumanendogenous retrovirus HERV-E (named CT-RCC HERV-E). In this study, we define a transcript encoding the entire envelope gene of HERV-E as expressed selectively in ccRCC tumors, as distinct from normal kidney tissues or other tumor types. Sequence analysis of this envelope transcript revealed long open reading frames encoding putative surface and transmembrane envelope proteins. Retroviral envelopes are known to be capable of eliciting immunity in humans. Accordingly, we found that HLA-A∗0201-restricted peptides predicted to be products of the CT-RCC HERV-E envelope transcript-stimulated CD8+ T cells, which could recognize HLA-A∗0201-positive HERV-E- expressing kidney tumor cells. Overall, our results offer evidence of unique HERV-E envelope peptides presented on the surface of ccRCC cells, offering potentially useful tumor-restricted targets for T-cell-based immunotherapy of kidney cancer.
AB - VHL-deficient clear cell renal cell carcinomas (ccRCC), the most common form of kidney cancer, express transcripts derived from the novelhumanendogenous retrovirus HERV-E (named CT-RCC HERV-E). In this study, we define a transcript encoding the entire envelope gene of HERV-E as expressed selectively in ccRCC tumors, as distinct from normal kidney tissues or other tumor types. Sequence analysis of this envelope transcript revealed long open reading frames encoding putative surface and transmembrane envelope proteins. Retroviral envelopes are known to be capable of eliciting immunity in humans. Accordingly, we found that HLA-A∗0201-restricted peptides predicted to be products of the CT-RCC HERV-E envelope transcript-stimulated CD8+ T cells, which could recognize HLA-A∗0201-positive HERV-E- expressing kidney tumor cells. Overall, our results offer evidence of unique HERV-E envelope peptides presented on the surface of ccRCC cells, offering potentially useful tumor-restricted targets for T-cell-based immunotherapy of kidney cancer.
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U2 - 10.1158/0008-5472.CAN-15-3139
DO - 10.1158/0008-5472.CAN-15-3139
M3 - Article
C2 - 26862115
AN - SCOPUS:84970951907
SN - 0008-5472
VL - 76
SP - 2177
EP - 2185
JO - Cancer Research
JF - Cancer Research
IS - 8
ER -
