TY - JOUR
T1 - Detection of bone marrow infiltration of lymphoma cells by fluorescence in situ hybridization
AU - Ishizawa, Kenichi
AU - Obara, Yasuhiko
AU - Seki, Osamu
AU - Fujimaki, Shinichi
AU - Kameoka, Junichi
AU - Ichinohasama, Ryo
AU - Kanemitsu, Keiji
AU - Sasaki, Takeshi
AU - Kaku, Mitsuo
AU - Harigae, Hideo
N1 - Funding Information:
The authors thank Ms. C. Seino and Ms. S. Akita for their excellent technical assistance, and Dr. M. Watanabe and Dr. T. Moriya for reviewing bone marrow biopsy. This work was supported in part by grants from the Ministry of Education, Science, Sports and Culture of Japan (OY and HH), and a Grant-in-Aid from Kurokawa Cancer Research Foundation (H.H.).
PY - 2004/6
Y1 - 2004/6
N2 - Background: It is sometimes difficult to detect the bone marrow infiltration of lymphoma cells, because lymphoma cells are not distinguishable from normal lymphocytes due to the similarity of their phenotype. Methods: Bone marrow involvement of 17 samples of 15 patients with follicular lymphoma, whose lymphoma cells were confirmed to harbor the translocation of chromosome14q32, were examined by microscopic analysis of bone marrow smear and biopsy, flow cytometorical analysis (FCM), chromosomal analysis of G-banding and fluorescence in situ hybridization (FISH). FISH was performed using a probe, which detects the split of IGH gene on 14q32. Results: The positivity of FISH was highest among these methods and FISH was able to detect the bone marrow involvement in one case who was defined as negative by bone marrow biopsy. Conclusions: FISH can be used for detection of bone marrow involvement of malignant lymphoma that carries chromosomal rearrangement involving 14q32.
AB - Background: It is sometimes difficult to detect the bone marrow infiltration of lymphoma cells, because lymphoma cells are not distinguishable from normal lymphocytes due to the similarity of their phenotype. Methods: Bone marrow involvement of 17 samples of 15 patients with follicular lymphoma, whose lymphoma cells were confirmed to harbor the translocation of chromosome14q32, were examined by microscopic analysis of bone marrow smear and biopsy, flow cytometorical analysis (FCM), chromosomal analysis of G-banding and fluorescence in situ hybridization (FISH). FISH was performed using a probe, which detects the split of IGH gene on 14q32. Results: The positivity of FISH was highest among these methods and FISH was able to detect the bone marrow involvement in one case who was defined as negative by bone marrow biopsy. Conclusions: FISH can be used for detection of bone marrow involvement of malignant lymphoma that carries chromosomal rearrangement involving 14q32.
KW - Bone marrow involvement
KW - Chromosome 14q32
KW - Fluorescence in situ hybridization
KW - IgH
KW - Malignant lymphoma
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U2 - 10.1016/j.cccn.2004.02.014
DO - 10.1016/j.cccn.2004.02.014
M3 - Article
C2 - 15149874
AN - SCOPUS:2442602494
SN - 0009-8981
VL - 344
SP - 79
EP - 82
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
IS - 1-2
ER -