Detection of diatoms in blood by a combination of membrane filtering and chemical digestion

Masato Funayama; Yasuhiro Aoki; Ismail M. Sebetan; Kaoru Sagisaka

doi:10.1016/0379-0738(87)90071-5

Detection of diatoms in blood by a combination of membrane filtering and chemical digestion

Masato Funayama, Yasuhiro Aoki, Ismail M. Sebetan, Kaoru Sagisaka

MED - Public Health

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

An improved method for detecting diatom in blood is reported. Blood of cadavers was obtained by cardiac puncture at inquest or from the left atrium directly at autopsy. The blood was hemolyzed by sodium dodecyl sulfate (SDS) and filtered through membrane filter (47 mm in diameter and 5 μm in pore size). When the blood was putrefied, two or three pieces of membranes filters were needed because of choking membrane pore. The membrane filters were digested with fuming nitric acid and diluted with distilled water. The diluted solution was filtered through membrane filter (25 mm in diameter) again. After drying the membrane filter was immersed in oil. Diatoms on the membrane filter were clearly observed microscopically.

Original language	English
Pages (from-to)	175-182
Number of pages	8
Journal	Forensic Science International
Volume	34
Issue number	3
DOIs	https://doi.org/10.1016/0379-0738(87)90071-5
Publication status	Published - 1987 Jul

Keywords

Diatom test
Drowning
Membrane filtering

ASJC Scopus subject areas

Pathology and Forensic Medicine

Access to Document

10.1016/0379-0738(87)90071-5

Cite this

@article{964a6c2b9c0a4e009e583ae699e7eeda,

title = "Detection of diatoms in blood by a combination of membrane filtering and chemical digestion",

abstract = "An improved method for detecting diatom in blood is reported. Blood of cadavers was obtained by cardiac puncture at inquest or from the left atrium directly at autopsy. The blood was hemolyzed by sodium dodecyl sulfate (SDS) and filtered through membrane filter (47 mm in diameter and 5 μm in pore size). When the blood was putrefied, two or three pieces of membranes filters were needed because of choking membrane pore. The membrane filters were digested with fuming nitric acid and diluted with distilled water. The diluted solution was filtered through membrane filter (25 mm in diameter) again. After drying the membrane filter was immersed in oil. Diatoms on the membrane filter were clearly observed microscopically.",

keywords = "Diatom test, Drowning, Membrane filtering",

author = "Masato Funayama and Yasuhiro Aoki and Sebetan, {Ismail M.} and Kaoru Sagisaka",

year = "1987",

month = jul,

doi = "10.1016/0379-0738(87)90071-5",

language = "English",

volume = "34",

pages = "175--182",

journal = "Forensic Science International",

issn = "0379-0738",

publisher = "Elsevier Ireland Ltd",

number = "3",

}

TY - JOUR

T1 - Detection of diatoms in blood by a combination of membrane filtering and chemical digestion

AU - Funayama, Masato

AU - Aoki, Yasuhiro

AU - Sebetan, Ismail M.

AU - Sagisaka, Kaoru

PY - 1987/7

Y1 - 1987/7

N2 - An improved method for detecting diatom in blood is reported. Blood of cadavers was obtained by cardiac puncture at inquest or from the left atrium directly at autopsy. The blood was hemolyzed by sodium dodecyl sulfate (SDS) and filtered through membrane filter (47 mm in diameter and 5 μm in pore size). When the blood was putrefied, two or three pieces of membranes filters were needed because of choking membrane pore. The membrane filters were digested with fuming nitric acid and diluted with distilled water. The diluted solution was filtered through membrane filter (25 mm in diameter) again. After drying the membrane filter was immersed in oil. Diatoms on the membrane filter were clearly observed microscopically.

AB - An improved method for detecting diatom in blood is reported. Blood of cadavers was obtained by cardiac puncture at inquest or from the left atrium directly at autopsy. The blood was hemolyzed by sodium dodecyl sulfate (SDS) and filtered through membrane filter (47 mm in diameter and 5 μm in pore size). When the blood was putrefied, two or three pieces of membranes filters were needed because of choking membrane pore. The membrane filters were digested with fuming nitric acid and diluted with distilled water. The diluted solution was filtered through membrane filter (25 mm in diameter) again. After drying the membrane filter was immersed in oil. Diatoms on the membrane filter were clearly observed microscopically.

KW - Diatom test

KW - Drowning

KW - Membrane filtering

UR - http://www.scopus.com/inward/record.url?scp=0023218309&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023218309&partnerID=8YFLogxK

U2 - 10.1016/0379-0738(87)90071-5

DO - 10.1016/0379-0738(87)90071-5

M3 - Article

C2 - 3666621

AN - SCOPUS:0023218309

SN - 0379-0738

VL - 34

SP - 175

EP - 182

JO - Forensic Science International

JF - Forensic Science International

IS - 3

ER -

Detection of diatoms in blood by a combination of membrane filtering and chemical digestion

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this