Detection of immunoreactive endothelin in plasma of hemodialysis patients

Kazuhito Totsune, Toraichi Mouri, Kazuhiro Takahashi, Minoru Ohneda, Masahiko Sone, Takao Saito, Kaoru Yoshinaga

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Two types of radioimmunoassay (RIA) methods for measuring endothelin (ET) in human plasma were developed. One was an extraction procedure using a Sep-Pak C18 cartridge, the other being a direct method. By the extraction method, plasma ET levels were lower than the detectable limit (7 pg/ml) in normal subjects and elevated in hemodialysis patients. The absolute values obtained via the direct method were 20-times higher than those from extraction. Gel-filtration experiments revealed that this discrepancy was mainly due to immunoreactive (IR-) endothelin-like substances of high molecular mass near 11.6 kDa (large IR-ET). Extraction of the peptide by the C18 cartridge could eliminate interference by large IR-ET and is important in the accurate measurement of ET concentrations in plasma.

Original languageEnglish
Pages (from-to)239-242
Number of pages4
JournalFEBS Letters
Issue number2
Publication statusPublished - 1989 Jun 5


  • (Human plasma)
  • Endothelin
  • Hemodialysis
  • Immunoreactivity
  • Radioimmunoassay


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