Detection of microspotted carcinoembryonic antigen on a glass substrate by scanning electrochemical microscopy

Hitoshi Shiku; Tomokazu Matsue; Isamu Uchida

doi:10.1021/ac950824c

Detection of microspotted carcinoembryonic antigen on a glass substrate by scanning electrochemical microscopy

Hitoshi Shiku, Tomokazu Matsue, Isamu Uchida

Tohoku University

Research output: Contribution to journal › Article › peer-review

103 Citations (Scopus)

Abstract

Microspots of carbinoembryonic antigen (CEA) on glass substrates were characterized by scanning electrochemical microscopy (SECM). CEA was immobilized via a sandiwch method using horseradish peroxidase (HRP)-labeled anti-CEA. The reduction current of the oxidized form of ferrocenylmethanol generated by the HRP reaction was monitored to view SECM images. This method detects as low as ∼10⁴ CEA molecules in a single 20-μm-radius spot.

Original language	English
Pages (from-to)	1276-1278
Number of pages	3
Journal	Analytical Chemistry
Volume	68
Issue number	7
DOIs	https://doi.org/10.1021/ac950824c
Publication status	Published - 1996

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title = "Detection of microspotted carcinoembryonic antigen on a glass substrate by scanning electrochemical microscopy",

abstract = "Microspots of carbinoembryonic antigen (CEA) on glass substrates were characterized by scanning electrochemical microscopy (SECM). CEA was immobilized via a sandiwch method using horseradish peroxidase (HRP)-labeled anti-CEA. The reduction current of the oxidized form of ferrocenylmethanol generated by the HRP reaction was monitored to view SECM images. This method detects as low as ∼104 CEA molecules in a single 20-μm-radius spot.",

author = "Hitoshi Shiku and Tomokazu Matsue and Isamu Uchida",

year = "1996",

doi = "10.1021/ac950824c",

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AU - Shiku, Hitoshi

AU - Matsue, Tomokazu

AU - Uchida, Isamu

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N2 - Microspots of carbinoembryonic antigen (CEA) on glass substrates were characterized by scanning electrochemical microscopy (SECM). CEA was immobilized via a sandiwch method using horseradish peroxidase (HRP)-labeled anti-CEA. The reduction current of the oxidized form of ferrocenylmethanol generated by the HRP reaction was monitored to view SECM images. This method detects as low as ∼104 CEA molecules in a single 20-μm-radius spot.

AB - Microspots of carbinoembryonic antigen (CEA) on glass substrates were characterized by scanning electrochemical microscopy (SECM). CEA was immobilized via a sandiwch method using horseradish peroxidase (HRP)-labeled anti-CEA. The reduction current of the oxidized form of ferrocenylmethanol generated by the HRP reaction was monitored to view SECM images. This method detects as low as ∼104 CEA molecules in a single 20-μm-radius spot.

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Detection of microspotted carcinoembryonic antigen on a glass substrate by scanning electrochemical microscopy

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