Detection of protein-protein interactions on SiO2/Si surfaces by spectroscopic ellipsometry

Seigo Kodera; Toshihide Okajima; Hidehiko Iwabuki; Daisuke Kitaguchi; Shun'ichi Kuroda; Tatsuo Yoshinobu; Katsuyuki Tanizawa; Masamitsu Futai; Hiroshi Iwasaki

doi:10.1016/S0003-2697(03)00422-6

Detection of protein-protein interactions on SiO₂/Si surfaces by spectroscopic ellipsometry

Seigo Kodera, Toshihide Okajima, Hidehiko Iwabuki, Daisuke Kitaguchi, Shun'ichi Kuroda, Tatsuo Yoshinobu, Katsuyuki Tanizawa, Masamitsu Futai, Hiroshi Iwasaki

MEN - Biomedical Engineering

Osaka University

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

We have applied spectroscopic ellipsometry to sensitive detection of specific protein-protein interactions on SiO₂/Si substrates. First, the change of ellipticity of the reflected polarized light (600-1100nm) was correlated with the thickness of the protein layer immobilized on SiO ₂/Si surfaces by measuring monomeric (myoglobin) and homotetrameric (hemoglobin) proteins with a similar monomer size. Protein-protein interactions were then measured with the antigen/antibody and cell-surface receptor/ligand systems; in each system either of the two proteins was bound to SiO ₂/Si substrates. Consequently, significant ellipticity changes were observed only for the cases where the interactions were specific. A specific antibody binding was also detectable with an antigen displayed on the surface of bacteriophage particles. These results show the usefulness of spectroscopic ellipsometry for sensitive detection of protein-protein interactions and its applicability to a detection method for the protein-based biochips to be developed in the future.

Original language	English
Pages (from-to)	65-70
Number of pages	6
Journal	Analytical Biochemistry
Volume	321
Issue number	1
DOIs	https://doi.org/10.1016/S0003-2697(03)00422-6
Publication status	Published - 2003 Oct 1

Keywords

Biochips
Protein-protein interaction
Sensitive detection
Spectroscopic ellipsometry

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10.1016/S0003-2697(03)00422-6

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title = "Detection of protein-protein interactions on SiO2/Si surfaces by spectroscopic ellipsometry",

abstract = "We have applied spectroscopic ellipsometry to sensitive detection of specific protein-protein interactions on SiO2/Si substrates. First, the change of ellipticity of the reflected polarized light (600-1100nm) was correlated with the thickness of the protein layer immobilized on SiO 2/Si surfaces by measuring monomeric (myoglobin) and homotetrameric (hemoglobin) proteins with a similar monomer size. Protein-protein interactions were then measured with the antigen/antibody and cell-surface receptor/ligand systems; in each system either of the two proteins was bound to SiO 2/Si substrates. Consequently, significant ellipticity changes were observed only for the cases where the interactions were specific. A specific antibody binding was also detectable with an antigen displayed on the surface of bacteriophage particles. These results show the usefulness of spectroscopic ellipsometry for sensitive detection of protein-protein interactions and its applicability to a detection method for the protein-based biochips to be developed in the future.",

keywords = "Biochips, Protein-protein interaction, Sensitive detection, Spectroscopic ellipsometry",

author = "Seigo Kodera and Toshihide Okajima and Hidehiko Iwabuki and Daisuke Kitaguchi and Shun'ichi Kuroda and Tatsuo Yoshinobu and Katsuyuki Tanizawa and Masamitsu Futai and Hiroshi Iwasaki",

note = "Funding Information: This work was supported by the Center-of-Excellence Program (No. 09CE2005) from the Ministry of Education, Science, Sports and Culture of Japan (to K.T., M.F., and H.I.) and a research grant for promotion of young scientists from the Institute of Scientific and Industrial Research, Osaka University (to T.O. and T.Y.). ",

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doi = "10.1016/S0003-2697(03)00422-6",

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T1 - Detection of protein-protein interactions on SiO2/Si surfaces by spectroscopic ellipsometry

AU - Kodera, Seigo

AU - Okajima, Toshihide

AU - Iwabuki, Hidehiko

AU - Kitaguchi, Daisuke

AU - Kuroda, Shun'ichi

AU - Yoshinobu, Tatsuo

AU - Tanizawa, Katsuyuki

AU - Futai, Masamitsu

AU - Iwasaki, Hiroshi

N1 - Funding Information: This work was supported by the Center-of-Excellence Program (No. 09CE2005) from the Ministry of Education, Science, Sports and Culture of Japan (to K.T., M.F., and H.I.) and a research grant for promotion of young scientists from the Institute of Scientific and Industrial Research, Osaka University (to T.O. and T.Y.).

PY - 2003/10/1

Y1 - 2003/10/1

N2 - We have applied spectroscopic ellipsometry to sensitive detection of specific protein-protein interactions on SiO2/Si substrates. First, the change of ellipticity of the reflected polarized light (600-1100nm) was correlated with the thickness of the protein layer immobilized on SiO 2/Si surfaces by measuring monomeric (myoglobin) and homotetrameric (hemoglobin) proteins with a similar monomer size. Protein-protein interactions were then measured with the antigen/antibody and cell-surface receptor/ligand systems; in each system either of the two proteins was bound to SiO 2/Si substrates. Consequently, significant ellipticity changes were observed only for the cases where the interactions were specific. A specific antibody binding was also detectable with an antigen displayed on the surface of bacteriophage particles. These results show the usefulness of spectroscopic ellipsometry for sensitive detection of protein-protein interactions and its applicability to a detection method for the protein-based biochips to be developed in the future.

AB - We have applied spectroscopic ellipsometry to sensitive detection of specific protein-protein interactions on SiO2/Si substrates. First, the change of ellipticity of the reflected polarized light (600-1100nm) was correlated with the thickness of the protein layer immobilized on SiO 2/Si surfaces by measuring monomeric (myoglobin) and homotetrameric (hemoglobin) proteins with a similar monomer size. Protein-protein interactions were then measured with the antigen/antibody and cell-surface receptor/ligand systems; in each system either of the two proteins was bound to SiO 2/Si substrates. Consequently, significant ellipticity changes were observed only for the cases where the interactions were specific. A specific antibody binding was also detectable with an antigen displayed on the surface of bacteriophage particles. These results show the usefulness of spectroscopic ellipsometry for sensitive detection of protein-protein interactions and its applicability to a detection method for the protein-based biochips to be developed in the future.

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KW - Sensitive detection

KW - Spectroscopic ellipsometry

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Detection of protein-protein interactions on SiO₂/Si surfaces by spectroscopic ellipsometry

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