Determination of active diaphorase immobilized at electrode surfaces

Hiroshi Yamada, Hitoshi Shiku, Tomokazu Matsue, Isamu Uchida

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


The activity of diaphorase (from Bacillus stearothermophilus) immobilized on glassy carbon (GC) electrodes was determined by analyzing the catalytic currents for oxidation of the immobilized enzyme using digital simulation techniques, which gives the concentration of the active enzyme at the electrode surface. Results show that the immobilization by the cross-linking reaction with glutaraldehyde deactivates the enzyme and only about 10% of the total enzyme remains active at the electrode surface.

Original languageEnglish
Pages (from-to)337-346
Number of pages10
JournalBioelectrochemistry and Bioenergetics
Issue number3
Publication statusPublished - 1993 Feb


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