Determination of chlorhexidine (CHD) and nonylphenolethoxylates (NPEOn) using LC-ESI-MS method and application to hemolyzed blood

Kiyotaka Usui; Takanori Hishinuma; Hiroaki Yamaguchi; Naoki Tachiiri; Junichi Goto

doi:10.1016/j.jchromb.2005.11.051

Determination of chlorhexidine (CHD) and nonylphenolethoxylates (NPEOn) using LC-ESI-MS method and application to hemolyzed blood

Kiyotaka Usui, Takanori Hishinuma, Hiroaki Yamaguchi, Naoki Tachiiri, Junichi Goto

MED - Public Health

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Rapid and reliable methods for identification of chlorhexidine (CHD) and nonylphenolethoxylates (NPEOn) in antiseptic and hemolyzed blood using electrospray ionization mass spectrometry (ESI-MS) were developed. Fragmental analysis provides accurate evidence for the presence of CHD in the samples. For the determination of CHD in hemolyzed blood, the method was also developed using LC-ESI-MS. Linearity of calibration curve was obtained over the concentration range of 0.1-11 μg/mL with residuals from -4.3 to 6.7%. We applied the methods to the case of suicidal injection of antiseptic and successfully detected CHD and NPEOn from hemolyzed blood. The CHD concentration was 352 μg/mL.

Original language	English
Pages (from-to)	105-109
Number of pages	5
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	831
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jchromb.2005.11.051
Publication status	Published - 2006 Feb 2

Keywords

Antiseptic
Blood
Chlorhexidine
Disinfectant
Forensic medicine
Forensic science
Human
LC-ESI
LC-ESI-MS
Nonylphenolethoxylates
Suicide
Toxicology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jchromb.2005.11.051

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title = "Determination of chlorhexidine (CHD) and nonylphenolethoxylates (NPEOn) using LC-ESI-MS method and application to hemolyzed blood",

abstract = "Rapid and reliable methods for identification of chlorhexidine (CHD) and nonylphenolethoxylates (NPEOn) in antiseptic and hemolyzed blood using electrospray ionization mass spectrometry (ESI-MS) were developed. Fragmental analysis provides accurate evidence for the presence of CHD in the samples. For the determination of CHD in hemolyzed blood, the method was also developed using LC-ESI-MS. Linearity of calibration curve was obtained over the concentration range of 0.1-11 μg/mL with residuals from -4.3 to 6.7%. We applied the methods to the case of suicidal injection of antiseptic and successfully detected CHD and NPEOn from hemolyzed blood. The CHD concentration was 352 μg/mL.",

keywords = "Antiseptic, Blood, Chlorhexidine, Disinfectant, Forensic medicine, Forensic science, Human, LC-ESI, LC-ESI-MS, Nonylphenolethoxylates, Suicide, Toxicology",

author = "Kiyotaka Usui and Takanori Hishinuma and Hiroaki Yamaguchi and Naoki Tachiiri and Junichi Goto",

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doi = "10.1016/j.jchromb.2005.11.051",

language = "English",

volume = "831",

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Determination of chlorhexidine (CHD) and nonylphenolethoxylates (NPEOn) using LC-ESI-MS method and application to hemolyzed blood. / Usui, Kiyotaka; Hishinuma, Takanori; Yamaguchi, Hiroaki et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 831, No. 1-2, 02.02.2006, p. 105-109.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Determination of chlorhexidine (CHD) and nonylphenolethoxylates (NPEOn) using LC-ESI-MS method and application to hemolyzed blood

AU - Usui, Kiyotaka

AU - Hishinuma, Takanori

AU - Yamaguchi, Hiroaki

AU - Tachiiri, Naoki

AU - Goto, Junichi

PY - 2006/2/2

Y1 - 2006/2/2

N2 - Rapid and reliable methods for identification of chlorhexidine (CHD) and nonylphenolethoxylates (NPEOn) in antiseptic and hemolyzed blood using electrospray ionization mass spectrometry (ESI-MS) were developed. Fragmental analysis provides accurate evidence for the presence of CHD in the samples. For the determination of CHD in hemolyzed blood, the method was also developed using LC-ESI-MS. Linearity of calibration curve was obtained over the concentration range of 0.1-11 μg/mL with residuals from -4.3 to 6.7%. We applied the methods to the case of suicidal injection of antiseptic and successfully detected CHD and NPEOn from hemolyzed blood. The CHD concentration was 352 μg/mL.

AB - Rapid and reliable methods for identification of chlorhexidine (CHD) and nonylphenolethoxylates (NPEOn) in antiseptic and hemolyzed blood using electrospray ionization mass spectrometry (ESI-MS) were developed. Fragmental analysis provides accurate evidence for the presence of CHD in the samples. For the determination of CHD in hemolyzed blood, the method was also developed using LC-ESI-MS. Linearity of calibration curve was obtained over the concentration range of 0.1-11 μg/mL with residuals from -4.3 to 6.7%. We applied the methods to the case of suicidal injection of antiseptic and successfully detected CHD and NPEOn from hemolyzed blood. The CHD concentration was 352 μg/mL.

KW - Antiseptic

KW - Blood

KW - Chlorhexidine

KW - Disinfectant

KW - Forensic medicine

KW - Forensic science

KW - Human

KW - LC-ESI

KW - LC-ESI-MS

KW - Nonylphenolethoxylates

KW - Suicide

KW - Toxicology

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JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 1-2

ER -

Determination of chlorhexidine (CHD) and nonylphenolethoxylates (NPEOn) using LC-ESI-MS method and application to hemolyzed blood

Abstract

Keywords

UN SDGs

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