Determination of organophosphorus pesticides in biological samples of acute poisoning by HPLC with diode-array detector

Yuko Cho; Nancy Matsuoka; Akira Kamiya

doi:10.1248/cpb.45.737

Determination of organophosphorus pesticides in biological samples of acute poisoning by HPLC with diode-array detector

Yuko Cho, Nancy Matsuoka, Akira Kamiya

AGR - Agricultural Chemistry

Yamaguchi University

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

We have developed a simple and rapid method for measuring 11 organophosphorus pesticides (dichlorvos, methidathion, salithion, malathion, fenitrothion, fenthion, parathion, diazinon, ethylthiometon, O-ethyl O-(4- nitrophenyl)phenylphosphonothioate (EPN) and chlorpyrifos) and one metabolite (3-methyl-4-nitrophenol) of fenitrothion in serum and urine of acute poisoning patients by HPLC with a diode-array detector. An aliquot of the biological sample after deproteinization by acetonitrile was injected into a C₁₈ column using acetonitrile-water as a mobile phase. The detection limits in serum and urine ranged from 0.05 to 6.8 μg/ml at a wavelength of 230 nm. This method was successfully applied to two actual cases of acute poisoning.

Original language	English
Pages (from-to)	737-740
Number of pages	4
Journal	Chemical and Pharmaceutical Bulletin
Volume	45
Issue number	4
DOIs	https://doi.org/10.1248/cpb.45.737
Publication status	Published - 1997 Apr

Keywords

HPLC diode-array
acute poisoning
organophosphorus pesticide
serum
urine

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10.1248/cpb.45.737

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N2 - We have developed a simple and rapid method for measuring 11 organophosphorus pesticides (dichlorvos, methidathion, salithion, malathion, fenitrothion, fenthion, parathion, diazinon, ethylthiometon, O-ethyl O-(4- nitrophenyl)phenylphosphonothioate (EPN) and chlorpyrifos) and one metabolite (3-methyl-4-nitrophenol) of fenitrothion in serum and urine of acute poisoning patients by HPLC with a diode-array detector. An aliquot of the biological sample after deproteinization by acetonitrile was injected into a C18 column using acetonitrile-water as a mobile phase. The detection limits in serum and urine ranged from 0.05 to 6.8 μg/ml at a wavelength of 230 nm. This method was successfully applied to two actual cases of acute poisoning.

AB - We have developed a simple and rapid method for measuring 11 organophosphorus pesticides (dichlorvos, methidathion, salithion, malathion, fenitrothion, fenthion, parathion, diazinon, ethylthiometon, O-ethyl O-(4- nitrophenyl)phenylphosphonothioate (EPN) and chlorpyrifos) and one metabolite (3-methyl-4-nitrophenol) of fenitrothion in serum and urine of acute poisoning patients by HPLC with a diode-array detector. An aliquot of the biological sample after deproteinization by acetonitrile was injected into a C18 column using acetonitrile-water as a mobile phase. The detection limits in serum and urine ranged from 0.05 to 6.8 μg/ml at a wavelength of 230 nm. This method was successfully applied to two actual cases of acute poisoning.

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KW - urine

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Determination of organophosphorus pesticides in biological samples of acute poisoning by HPLC with diode-array detector

Abstract

Keywords

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