Determination of phosphatidylcholine hydroperoxide in human plasma by chemiluminescence - high performance liquid chromatography

Teruo Miyazawa; Keiichi Yasuda; Kenshiro Fujimoto; Takashi Kaneda

doi:10.1080/00032718808071929

Determination of phosphatidylcholine hydroperoxide in human plasma by chemiluminescence - high performance liquid chromatography

Teruo Miyazawa, Keiichi Yasuda, Kenshiro Fujimoto, Takashi Kaneda

New Industry Creation Hatchery Center (NICHe)

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

To determine phosphatidylcholine hydroperoxide (PCOOH) in human plasma lipids, a chemiluminescence - high performance liquid chromatography (CL-HPLC)system was newly developed and applied. This system consisted of separation of phosphatidylcholine derivatives with normal phase HPLC and detection of hydroperoxide-specific chemiluminescence of PCOOH. By use of this system, the PCOOH in normal human plasma was quantitatively assayed to be 28–431 pmol hydroperoxide-0₂ per ml of plasma. The high sensitivities, reproducibilities and specificities of the CL-HPLC assay were shown for determining plasma PCOOH.

Original language	English
Pages (from-to)	1033-1044
Number of pages	12
Journal	Analytical Letters
Volume	21
Issue number	6
DOIs	https://doi.org/10.1080/00032718808071929
Publication status	Published - 1988 Jun 1

Keywords

Chemiluminescence
HPLC
human plasma
phosphatidylcholine hydroperoxide

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Spectroscopy
Clinical Biochemistry
Biochemistry, medical
Electrochemistry

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10.1080/00032718808071929

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abstract = "To determine phosphatidylcholine hydroperoxide (PCOOH) in human plasma lipids, a chemiluminescence - high performance liquid chromatography (CL-HPLC)system was newly developed and applied. This system consisted of separation of phosphatidylcholine derivatives with normal phase HPLC and detection of hydroperoxide-specific chemiluminescence of PCOOH. By use of this system, the PCOOH in normal human plasma was quantitatively assayed to be 28–431 pmol hydroperoxide-02 per ml of plasma. The high sensitivities, reproducibilities and specificities of the CL-HPLC assay were shown for determining plasma PCOOH.",

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T1 - Determination of phosphatidylcholine hydroperoxide in human plasma by chemiluminescence - high performance liquid chromatography

AU - Miyazawa, Teruo

AU - Yasuda, Keiichi

AU - Fujimoto, Kenshiro

AU - Kaneda, Takashi

PY - 1988/6/1

Y1 - 1988/6/1

N2 - To determine phosphatidylcholine hydroperoxide (PCOOH) in human plasma lipids, a chemiluminescence - high performance liquid chromatography (CL-HPLC)system was newly developed and applied. This system consisted of separation of phosphatidylcholine derivatives with normal phase HPLC and detection of hydroperoxide-specific chemiluminescence of PCOOH. By use of this system, the PCOOH in normal human plasma was quantitatively assayed to be 28–431 pmol hydroperoxide-02 per ml of plasma. The high sensitivities, reproducibilities and specificities of the CL-HPLC assay were shown for determining plasma PCOOH.

AB - To determine phosphatidylcholine hydroperoxide (PCOOH) in human plasma lipids, a chemiluminescence - high performance liquid chromatography (CL-HPLC)system was newly developed and applied. This system consisted of separation of phosphatidylcholine derivatives with normal phase HPLC and detection of hydroperoxide-specific chemiluminescence of PCOOH. By use of this system, the PCOOH in normal human plasma was quantitatively assayed to be 28–431 pmol hydroperoxide-02 per ml of plasma. The high sensitivities, reproducibilities and specificities of the CL-HPLC assay were shown for determining plasma PCOOH.

KW - Chemiluminescence

KW - HPLC

KW - human plasma

KW - phosphatidylcholine hydroperoxide

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Determination of phosphatidylcholine hydroperoxide in human plasma by chemiluminescence - high performance liquid chromatography

Abstract

Keywords

ASJC Scopus subject areas

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