TY - JOUR
T1 - Determination of phospholipid hydroperoxides in human blood plasma by a chemiluminescence-HPLC assay
AU - Miyazawa, Teruo
PY - 1989
Y1 - 1989
N2 - A chemiluminiscence-high performance liquid chromatography (CL-HPLC) system was developed (Miyazawa, T. et al., Anal. Lett., 20, 915-925, 1987) and applied for the hydroperoxide-specific determination of phosphatidylcholine hydroperoxide (PCOOH) in biological tissues such as human blood plasma (Miyazawa, T. et al., Anal Lett 21: 1033-1044 1988; J. Biochem. 103: 744-746; 1988). This system involves separation of phosphatidylcholines from plasma total lipids with normal phase silica gel HPLC and post-column detection of hydroperoxide-dependent chemiluminescence of PCOOH. The chemiluminescence is produced by luminol oxidation during a reactor of hydroperoxide and cytochrome c-heme. The high specificity for hydroperoxide base enables a sensitive assay for a large range of PCOOH, with the detection limit of 10 picomole of hydroperoxide-O2. By use of this assay system, the presence of PCOOH in human blood plasma is confirmed quantitatively. The PCOOH concentration of healthy plasma is in the range below 10 nM to 500 nM, and much higher concentrations (500-9000 nM) of PCOOH are observed in the plasma of unhealthy donors.
AB - A chemiluminiscence-high performance liquid chromatography (CL-HPLC) system was developed (Miyazawa, T. et al., Anal. Lett., 20, 915-925, 1987) and applied for the hydroperoxide-specific determination of phosphatidylcholine hydroperoxide (PCOOH) in biological tissues such as human blood plasma (Miyazawa, T. et al., Anal Lett 21: 1033-1044 1988; J. Biochem. 103: 744-746; 1988). This system involves separation of phosphatidylcholines from plasma total lipids with normal phase silica gel HPLC and post-column detection of hydroperoxide-dependent chemiluminescence of PCOOH. The chemiluminescence is produced by luminol oxidation during a reactor of hydroperoxide and cytochrome c-heme. The high specificity for hydroperoxide base enables a sensitive assay for a large range of PCOOH, with the detection limit of 10 picomole of hydroperoxide-O2. By use of this assay system, the presence of PCOOH in human blood plasma is confirmed quantitatively. The PCOOH concentration of healthy plasma is in the range below 10 nM to 500 nM, and much higher concentrations (500-9000 nM) of PCOOH are observed in the plasma of unhealthy donors.
KW - Chemiluminescence
KW - Free radicals
KW - HPLC
KW - Lipid hydroperoxides
KW - Phosphatidylcholine hydroperoxide
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U2 - 10.1016/0891-5849(89)90017-8
DO - 10.1016/0891-5849(89)90017-8
M3 - Article
C2 - 2806945
AN - SCOPUS:0024451299
SN - 0891-5849
VL - 7
SP - 209
EP - 218
JO - Free Radical Biology and Medicine
JF - Free Radical Biology and Medicine
IS - 2
ER -
