TY - JOUR
T1 - Determination of Qx - and Qy - absorption bands of Zn-porphyrin derivatives contained in proteins by hole-burning spectroscopy
AU - Shibata, Yutaka
AU - Kushida, Takashi
N1 - Funding Information:
The authors express their gratitude to Prof. Y. Goto, Dr. D. Hamada, Prof. S. Aimoto, Miss K. Matsuda, and Miss R. Kaneko of Osaka University for their help in preparation of the ZnMP-cytC samples. This work was supported by the Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists.
PY - 1998/2/20
Y1 - 1998/2/20
N2 - The Qx- and Qy- absorption components of Zn-protoporphyrin and Zn-mesoporphyrin substituted respectively in myoglobin and cytochrome C have been extracted from inhomogeneously broadened absorption spectra by means of polarization-dependent transient hole-burning spectroscopy. The splitting between these components has been obtained as ~330 cm-1 and ~230 cm-1 for myoglobin and cytochrome C, respectively. The intensities of these two bands are much different in the case of myoglobin, while they have comparative intensities in the case of cytochrome C. It has been suggested that the porphyrin molecule feels much stronger local field in myoglobin than in cytochrome C.
AB - The Qx- and Qy- absorption components of Zn-protoporphyrin and Zn-mesoporphyrin substituted respectively in myoglobin and cytochrome C have been extracted from inhomogeneously broadened absorption spectra by means of polarization-dependent transient hole-burning spectroscopy. The splitting between these components has been obtained as ~330 cm-1 and ~230 cm-1 for myoglobin and cytochrome C, respectively. The intensities of these two bands are much different in the case of myoglobin, while they have comparative intensities in the case of cytochrome C. It has been suggested that the porphyrin molecule feels much stronger local field in myoglobin than in cytochrome C.
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U2 - 10.1016/s0009-2614(97)01366-3
DO - 10.1016/s0009-2614(97)01366-3
M3 - Article
AN - SCOPUS:0032548628
SN - 0009-2614
VL - 284
SP - 115
EP - 120
JO - Chemical Physics Letters
JF - Chemical Physics Letters
IS - 1-2
ER -