Determination of sphingolipids by LC-MS/MS

Tomohiro Takahashi; Chihiro Takeda; Kohei Saito; Naoto Suzuki; Hiroki Tsukamoto; Yoshihisa Tomioka

doi:10.1007/978-4-431-55669-5_26

Determination of sphingolipids by LC-MS/MS

Tomohiro Takahashi, Chihiro Takeda, Kohei Saito, Naoto Suzuki, Hiroki Tsukamoto, Yoshihisa Tomioka

PHA - Pharmacy

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

To investigate the biological functions and roles of sphingolipids, sensitive and compound-specific methods are required to measure their levels in biological samples. Liquid chromatography-mass spectrometry (LC-MS) using electrospray ionization (ESI) is suitable for the reliable simultaneous analysis of multiple compounds. In addition, the selected reaction monitoring (SRM) mode of tandem mass spectrometry (MS/MS) is effective to quantify with high sensitivity and selectivity. Therefore, LC-MS/MS came to be utilized for simultaneous analysis of the sphingolipids in vivo. Useful methods for the sphingolipids and related features are also summarized. The following protocol demonstrates information on determination of sphingolipids, especially sphingosine and sphingosine-1- phosphate, by LC-ESI-MS/MS in biological samples such as cell lysates, plasma, serum, orurine.

Original language	English
Title of host publication	Bioactive Lipid Mediators
Subtitle of host publication	Current Reviews and Protocols
Publisher	Springer Japan
Pages	357-370
Number of pages	14
ISBN (Electronic)	9784431556695
ISBN (Print)	9784431556688
DOIs	https://doi.org/10.1007/978-4-431-55669-5_26
Publication status	Published - 2015 Jan 1

Keywords

Dihydrosphingosine
Dihydrosphingosine-1-phosphate
LC-MS/MS
Phytosphingosine
Phytosphingosine-1-phosphate
Sphingolipids
Sphingosine
Sphingosine-1-phosphate

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Medicine(all)

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10.1007/978-4-431-55669-5_26

Cite this

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title = "Determination of sphingolipids by LC-MS/MS",

abstract = "To investigate the biological functions and roles of sphingolipids, sensitive and compound-specific methods are required to measure their levels in biological samples. Liquid chromatography-mass spectrometry (LC-MS) using electrospray ionization (ESI) is suitable for the reliable simultaneous analysis of multiple compounds. In addition, the selected reaction monitoring (SRM) mode of tandem mass spectrometry (MS/MS) is effective to quantify with high sensitivity and selectivity. Therefore, LC-MS/MS came to be utilized for simultaneous analysis of the sphingolipids in vivo. Useful methods for the sphingolipids and related features are also summarized. The following protocol demonstrates information on determination of sphingolipids, especially sphingosine and sphingosine-1- phosphate, by LC-ESI-MS/MS in biological samples such as cell lysates, plasma, serum, orurine.",

keywords = "Dihydrosphingosine, Dihydrosphingosine-1-phosphate, LC-MS/MS, Phytosphingosine, Phytosphingosine-1-phosphate, Sphingolipids, Sphingosine, Sphingosine-1-phosphate",

author = "Tomohiro Takahashi and Chihiro Takeda and Kohei Saito and Naoto Suzuki and Hiroki Tsukamoto and Yoshihisa Tomioka",

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T1 - Determination of sphingolipids by LC-MS/MS

AU - Takahashi, Tomohiro

AU - Takeda, Chihiro

AU - Saito, Kohei

AU - Suzuki, Naoto

AU - Tsukamoto, Hiroki

AU - Tomioka, Yoshihisa

N1 - Publisher Copyright: © Springer Japan 2015.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - To investigate the biological functions and roles of sphingolipids, sensitive and compound-specific methods are required to measure their levels in biological samples. Liquid chromatography-mass spectrometry (LC-MS) using electrospray ionization (ESI) is suitable for the reliable simultaneous analysis of multiple compounds. In addition, the selected reaction monitoring (SRM) mode of tandem mass spectrometry (MS/MS) is effective to quantify with high sensitivity and selectivity. Therefore, LC-MS/MS came to be utilized for simultaneous analysis of the sphingolipids in vivo. Useful methods for the sphingolipids and related features are also summarized. The following protocol demonstrates information on determination of sphingolipids, especially sphingosine and sphingosine-1- phosphate, by LC-ESI-MS/MS in biological samples such as cell lysates, plasma, serum, orurine.

AB - To investigate the biological functions and roles of sphingolipids, sensitive and compound-specific methods are required to measure their levels in biological samples. Liquid chromatography-mass spectrometry (LC-MS) using electrospray ionization (ESI) is suitable for the reliable simultaneous analysis of multiple compounds. In addition, the selected reaction monitoring (SRM) mode of tandem mass spectrometry (MS/MS) is effective to quantify with high sensitivity and selectivity. Therefore, LC-MS/MS came to be utilized for simultaneous analysis of the sphingolipids in vivo. Useful methods for the sphingolipids and related features are also summarized. The following protocol demonstrates information on determination of sphingolipids, especially sphingosine and sphingosine-1- phosphate, by LC-ESI-MS/MS in biological samples such as cell lysates, plasma, serum, orurine.

KW - Dihydrosphingosine

KW - Dihydrosphingosine-1-phosphate

KW - LC-MS/MS

KW - Phytosphingosine

KW - Phytosphingosine-1-phosphate

KW - Sphingolipids

KW - Sphingosine

KW - Sphingosine-1-phosphate

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M3 - Chapter

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SP - 357

EP - 370

BT - Bioactive Lipid Mediators

PB - Springer Japan

ER -

Determination of sphingolipids by LC-MS/MS

Abstract

Keywords

ASJC Scopus subject areas

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