TY - JOUR
T1 - Determination of urinary 12(S)-hydroxyeicosatetraenoic acid by liquid chromatography-tandem mass spectrometry with column-switching technique
T2 - Sex difference in healthy volunteers and patients with diabetes mellitus
AU - Suzuki, Naoto
AU - Hishinuma, Takanori
AU - Saga, Toshihide
AU - Sato, Jo
AU - Toyota, Takayoshi
AU - Goto, Junichi
AU - Mizugaki, Michinao
N1 - Funding Information:
This study was supported in part by grants from Uehara Memorial Foundation in Japan, the Health Sciences Research Grants for the Research on Pharmaceutical and Medical Safety from the Ministry of Health and Welfare of Japan, and Grants-in-Aid for the Research on Health Sciences Focusing on Drug Innovation from Japan Health Sciences Foundation.
PY - 2003/1/15
Y1 - 2003/1/15
N2 - We developed a determination method for human urinary 12-hydroxyeicosatetraenoic acid (12-HETE) using LC-MS-MS. This method, which includes simple extraction and detection in the SRM mode, allows precise and accurate determination of 12-HETE. There was a significant sex difference in urinary 12-HETE levels. Chiral analysis of 12-HETE using LC-MS-MS with column-switching technique revealed that the major enantiomer was 12(S)-HETE. Furthermore, the urinary level in patients with diabetes mellitus (DM) was analyzed. The present in vivo findings indicate that there could be difference in production of 12(S)-HETE between genders and 12(S)-HETE may play a role in the pathogenesis of DM.
AB - We developed a determination method for human urinary 12-hydroxyeicosatetraenoic acid (12-HETE) using LC-MS-MS. This method, which includes simple extraction and detection in the SRM mode, allows precise and accurate determination of 12-HETE. There was a significant sex difference in urinary 12-HETE levels. Chiral analysis of 12-HETE using LC-MS-MS with column-switching technique revealed that the major enantiomer was 12(S)-HETE. Furthermore, the urinary level in patients with diabetes mellitus (DM) was analyzed. The present in vivo findings indicate that there could be difference in production of 12(S)-HETE between genders and 12(S)-HETE may play a role in the pathogenesis of DM.
KW - 12(S)-Hydroxyeicosatetraenoic acid
UR - http://www.scopus.com/inward/record.url?scp=0037437629&partnerID=8YFLogxK
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U2 - 10.1016/S1570-0232(02)00666-9
DO - 10.1016/S1570-0232(02)00666-9
M3 - Article
C2 - 12482481
AN - SCOPUS:0037437629
SN - 1570-0232
VL - 783
SP - 383
EP - 389
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 2
ER -
