@article{a539c41fbfc9489f9e01b88926b71807,
title = "Development and characterization of EST-SSR markers for Carex angustisquama (Cyperaceae), an extremophyte in solfatara fields",
abstract = "Premise of the Study: Expressed sequence tag–simple sequence repeat (EST-SSR) markers were developed for Carex angustisquama (Cyperaceae) to investigate the evolutionary history of this plant that is endemic to solfatara fields in northern Japan. Methods and Results: Using RNA-Seq data generated by the Illumina HiSeq 2000, 20 EST-SSR markers were developed. Polymorphisms were assessed in C. angustisquama and the closely related species C. doenitzii and C. podogyna. In C. angustisquama, many loci were monomorphic within populations; the average number of alleles ranged from one to five, and levels of expected heterozygosity ranged from 0.000 to 0.580, while all markers were polymorphic in a population of C. doenitzii. This indicates that low genetic polymorphism of C. angustisquama is likely due to the species{\textquoteright} population dynamics, rather than to null alleles at the developed markers. Conclusions: These markers will be used to assess genetic diversity and structure and to investigate evolutionary history in future studies of C. angustisquama and related species.",
keywords = "Carex angustisquama, Carex doenitzii, Cyperaceae, expressed sequence tag–simple sequence repeat (EST-SSR) markers, solfatara",
author = "Koki Nagasawa and Hiroaki Setoguchi and Masayuki Maki and Hayato Goto and Keitaro Fukushima and Yuji Isagi and Shota Sakaguchi",
note = "Funding Information: The authors thank Mr. K. Sawa (Yamagata Prefecture), Mr. S. Kurata (University of Tokyo), Dr. Y. Suyama (Tohoku University), Dr. Y. Tsunamoto (Forest Research and Management Organization), and Dr. K. Yonekura (Tohoku University) for providing plant materials. We are also grateful to Dr. J. R. P. Worth for his comments on an earlier version of the manuscript. This research was supported by the Japan Society for the Promotion of Science (Bilateral program “The spatial and temporal dimensions and underlying mechanisms of lineage divergence and plant speciation of keystone species in Sino-Japanese Forest subkingdom” and Grant-in-Aid for Young Scientists B [no. 17K15286]), the SICORP Program of the Japan Science and Technology Agency (“Spatial-temporal dimensions and underlying mechanisms of lineage diversification and patterns of genetic variation of keystone plant taxa in warm-temperate forests of Sino-Japanese Floristic Region”; grant no. 4–1403), the TAKARA Harmonist Fund, the Nissei Fund, and the Pro Natura Foundation Japan for the Ashiu Biological Conservation project. Funding Information: The authors thank Mr. K. Sawa (Yamagata Prefecture), Mr. S. Kurata (University of Tokyo), Dr. Y. Suyama (Tohoku University), Dr. Y. Tsunamoto (Forest Research and Management Organization), and Dr. K. Yonekura (Tohoku University) for providing plant materials. We are also grateful to Dr. J. R. P. Worth for his comments on an earlier version of the manuscript. This research was supported by the Japan Society for the Promotion of Science (Bilateral program ?The spatial and temporal dimensions and underlying mechanisms of lineage divergence and plant speciation of keystone species in Sino-Japanese Forest subkingdom? and Grant-in-Aid for Young Scientists B [no. 17K15286]), the SICORP Program of the Japan Science and Technology Agency (?Spatial-temporal dimensions and underlying mechanisms of lineage diversification and patterns of genetic variation of keystone plant taxa in warm-temperate forests of Sino-Japanese Floristic Region?; grant no. 4?1403), the TAKARA Harmonist Fund, the Nissei Fund, and the Pro Natura Foundation Japan for the Ashiu Biological Conservation project. Publisher Copyright: {\textcopyright} 2018 Nagasawa et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America",
year = "2018",
month = oct,
doi = "10.1002/aps3.1185",
language = "English",
volume = "6",
journal = "Applications in Plant Sciences",
issn = "2168-0450",
publisher = "John Wiley & Sons Inc.",
number = "10",
}