TY - JOUR
T1 - Development and characterization of nine novel microsatellite markers for the milkfish Chanos chanos
AU - Santos, Brian S.
AU - Romana-Eguia, Maria Rowena R.
AU - Basiao, Zubaida U.
AU - Ikeda, Minoru
N1 - Funding Information:
Funds for this study were provided by the Department of Science and Technology-Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development (DOST-PCAARRD) and the Southeast Asian Fisheries Development Center, Aquaculture Department (SEAFDEC/AQD; study code Br-02-F2012B).
Publisher Copyright:
© 2014, Springer Science+Business Media Dordrecht.
PY - 2015/6/1
Y1 - 2015/6/1
N2 - The milkfish, Chanos chanos, is an important aquaculture resource in Southeast Asia. Using NGS data, 72 microsatellite markers were developed. PCR product confirmation using agarose gel electrophoresis allowed the identification of 24 utilizable markers. Milkfish samples (n = 48) from a hatchery stock in Palawan, Philippines were analyzed for genetic variability at the aforementioned 24 loci. Consequently, nine of these microsatellite loci were noted to have high success rate in amplification, exhibited polymorphism with 19 maximum number of alleles and no null alleles. These are the first microsatellite markers to be developed and characterized for C. chanos that will efficiently enable genetic stock delineation and monitoring as well as marker-aided genetic improvement research.
AB - The milkfish, Chanos chanos, is an important aquaculture resource in Southeast Asia. Using NGS data, 72 microsatellite markers were developed. PCR product confirmation using agarose gel electrophoresis allowed the identification of 24 utilizable markers. Milkfish samples (n = 48) from a hatchery stock in Palawan, Philippines were analyzed for genetic variability at the aforementioned 24 loci. Consequently, nine of these microsatellite loci were noted to have high success rate in amplification, exhibited polymorphism with 19 maximum number of alleles and no null alleles. These are the first microsatellite markers to be developed and characterized for C. chanos that will efficiently enable genetic stock delineation and monitoring as well as marker-aided genetic improvement research.
KW - Chanos chanos
KW - Microsatellite markers
KW - Milkfish
KW - Population genetic structure
UR - http://www.scopus.com/inward/record.url?scp=84928983308&partnerID=8YFLogxK
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U2 - 10.1007/s12686-014-0393-3
DO - 10.1007/s12686-014-0393-3
M3 - Letter
AN - SCOPUS:84928983308
SN - 1877-7252
VL - 7
SP - 451
EP - 453
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
IS - 2
ER -