Development of a crystallization protocol for the DbeAl variant of novel haloalkane dehalogenase from bradyrhizobium elkani USDA94

T. Prudnikova; R. Chaloupkova; Y. Sato; Y. Nagata; O. Degtjarik; M. Kuty; P. Rezacova; J. Damborsky; J. Kuta Smatanova

doi:10.1021/cg1013363

Development of a crystallization protocol for the DbeAl variant of novel haloalkane dehalogenase from bradyrhizobium elkani USDA94

T. Prudnikova, R. Chaloupkova, Y. Sato, Y. Nagata, O. Degtjarik, M. Kuty, P. Rezacova, J. Damborsky, J. Kuta Smatanova

LIF - Molecular and Chemical Life Science

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The DbeA1 variant of a novel haloalkane dehalogenase DbeA (EC 3.8.1.5) from Bradyrhizobium elkani USDA94 was constructed to study the structure-function relationships between DbjA and DbeA enzymes. A DbeA1 variant carries a unique fragment of nine amino acids transplanted from the sequentially closely related enzyme DbjA from Bradyrhizobium japonicum USDA110 to DbeA. Here we report development of the crystallization protocol for DbeA1 and soaking experiments with the ligands 1-fluoropentane and 1, 3-dichloropropane.

Original language	English
Pages (from-to)	516-519
Number of pages	4
Journal	Crystal Growth and Design
Volume	11
Issue number	2
DOIs	https://doi.org/10.1021/cg1013363
Publication status	Published - 2011 Feb 2

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abstract = "The DbeA1 variant of a novel haloalkane dehalogenase DbeA (EC 3.8.1.5) from Bradyrhizobium elkani USDA94 was constructed to study the structure-function relationships between DbjA and DbeA enzymes. A DbeA1 variant carries a unique fragment of nine amino acids transplanted from the sequentially closely related enzyme DbjA from Bradyrhizobium japonicum USDA110 to DbeA. Here we report development of the crystallization protocol for DbeA1 and soaking experiments with the ligands 1-fluoropentane and 1, 3-dichloropropane.",

author = "T. Prudnikova and R. Chaloupkova and Y. Sato and Y. Nagata and O. Degtjarik and M. Kuty and P. Rezacova and J. Damborsky and {Kuta Smatanova}, J.",

year = "2011",

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doi = "10.1021/cg1013363",

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T1 - Development of a crystallization protocol for the DbeAl variant of novel haloalkane dehalogenase from bradyrhizobium elkani USDA94

AU - Prudnikova, T.

AU - Chaloupkova, R.

AU - Sato, Y.

AU - Nagata, Y.

AU - Degtjarik, O.

AU - Kuty, M.

AU - Rezacova, P.

AU - Damborsky, J.

AU - Kuta Smatanova, J.

PY - 2011/2/2

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Development of a crystallization protocol for the DbeAl variant of novel haloalkane dehalogenase from bradyrhizobium elkani USDA94

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