Development of a method to detect viral RNA sequences from cultured cells by combining size fraction and a rapid determination system for viral RNA sequences (RDV)

Shumpei Watanabe; Tetsuya Mizutani; Kouji Sakai; Itoe Iizuka; Tomoyuki Shiota; Yusuke Sayama; Shumpei Tsuda; Kentaro Kato; Shuetsu Fukushi; Masayuki Saijo; Ichiro Kurane; Shigeru Morikawa; Hiroomi Akashi

doi:10.4172/2157-7579.1000103

Development of a method to detect viral RNA sequences from cultured cells by combining size fraction and a rapid determination system for viral RNA sequences (RDV)

Shumpei Watanabe, Tetsuya Mizutani, Kouji Sakai, Itoe Iizuka, Tomoyuki Shiota, Yusuke Sayama, Shumpei Tsuda, Kentaro Kato, Shuetsu Fukushi, Masayuki Saijo, Ichiro Kurane, Shigeru Morikawa, Hiroomi Akashi

Research output: Contribution to journal › Article › peer-review

Abstract

By combining size-fraction and a rapid determination system for viral RNA sequences (RDV), a method for sequence independent detection from virus-infected cultured cells was developed as "RDV-size fraction (RDV-SF)". Using RDV-SF, we succeeded in detecting nucleotide fragment sequences of feline calicivirus (FCV) and Yokose virus (YOKV) from Vero E6 cells infected with FCV and YOKV, respectively. RDV-SF was useful in identifying RNA viruses with a genome size of more than 5 kb from virus-infected cultured cells.

Original language	English
Journal	Journal of Veterinary Science and Technology
Volume	1
Issue number	1
DOIs	https://doi.org/10.4172/2157-7579.1000103
Publication status	Published - 2012

Keywords

Direct sequencing
RDV-SF
RNA virus
Sequence-independent detection

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10.4172/2157-7579.1000103

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Watanabe, S., Mizutani, T., Sakai, K., Iizuka, I., Shiota, T., Sayama, Y., Tsuda, S., Kato, K., Fukushi, S., Saijo, M., Kurane, I., Morikawa, S., & Akashi, H. (2012). Development of a method to detect viral RNA sequences from cultured cells by combining size fraction and a rapid determination system for viral RNA sequences (RDV). Journal of Veterinary Science and Technology, 1(1). https://doi.org/10.4172/2157-7579.1000103

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title = "Development of a method to detect viral RNA sequences from cultured cells by combining size fraction and a rapid determination system for viral RNA sequences (RDV)",

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keywords = "Direct sequencing, RDV-SF, RNA virus, Sequence-independent detection",

author = "Shumpei Watanabe and Tetsuya Mizutani and Kouji Sakai and Itoe Iizuka and Tomoyuki Shiota and Yusuke Sayama and Shumpei Tsuda and Kentaro Kato and Shuetsu Fukushi and Masayuki Saijo and Ichiro Kurane and Shigeru Morikawa and Hiroomi Akashi",

year = "2012",

doi = "10.4172/2157-7579.1000103",

language = "English",

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journal = "Journal of Veterinary Science and Technology",

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Watanabe, S, Mizutani, T, Sakai, K, Iizuka, I, Shiota, T, Sayama, Y, Tsuda, S, Kato, K, Fukushi, S, Saijo, M, Kurane, I, Morikawa, S & Akashi, H 2012, 'Development of a method to detect viral RNA sequences from cultured cells by combining size fraction and a rapid determination system for viral RNA sequences (RDV)', Journal of Veterinary Science and Technology, vol. 1, no. 1. https://doi.org/10.4172/2157-7579.1000103

TY - JOUR

T1 - Development of a method to detect viral RNA sequences from cultured cells by combining size fraction and a rapid determination system for viral RNA sequences (RDV)

AU - Watanabe, Shumpei

AU - Mizutani, Tetsuya

AU - Sakai, Kouji

AU - Iizuka, Itoe

AU - Shiota, Tomoyuki

AU - Sayama, Yusuke

AU - Tsuda, Shumpei

AU - Kato, Kentaro

AU - Fukushi, Shuetsu

AU - Saijo, Masayuki

AU - Kurane, Ichiro

AU - Morikawa, Shigeru

AU - Akashi, Hiroomi

PY - 2012

Y1 - 2012

N2 - By combining size-fraction and a rapid determination system for viral RNA sequences (RDV), a method for sequence independent detection from virus-infected cultured cells was developed as "RDV-size fraction (RDV-SF)". Using RDV-SF, we succeeded in detecting nucleotide fragment sequences of feline calicivirus (FCV) and Yokose virus (YOKV) from Vero E6 cells infected with FCV and YOKV, respectively. RDV-SF was useful in identifying RNA viruses with a genome size of more than 5 kb from virus-infected cultured cells.

AB - By combining size-fraction and a rapid determination system for viral RNA sequences (RDV), a method for sequence independent detection from virus-infected cultured cells was developed as "RDV-size fraction (RDV-SF)". Using RDV-SF, we succeeded in detecting nucleotide fragment sequences of feline calicivirus (FCV) and Yokose virus (YOKV) from Vero E6 cells infected with FCV and YOKV, respectively. RDV-SF was useful in identifying RNA viruses with a genome size of more than 5 kb from virus-infected cultured cells.

KW - Direct sequencing

KW - RDV-SF

KW - RNA virus

KW - Sequence-independent detection

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U2 - 10.4172/2157-7579.1000103

DO - 10.4172/2157-7579.1000103

M3 - Article

AN - SCOPUS:84883550783

SN - 2157-7579

VL - 1

JO - Journal of Veterinary Science and Technology

JF - Journal of Veterinary Science and Technology

IS - 1

ER -

Development of a method to detect viral RNA sequences from cultured cells by combining size fraction and a rapid determination system for viral RNA sequences (RDV)

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