TY - JOUR
T1 - Development of an anti-bear podoplanin monoclonal antibody PMab-247 for immunohistochemical analysis
AU - Furusawa, Yoshikazu
AU - Takei, Junko
AU - Sayama, Yusuke
AU - Yamada, Shinji
AU - Kaneko, Mika K.
AU - Kato, Yukinari
N1 - Funding Information:
This research was supported in part by AMED under Grant Numbers: JP18am0101078 (Y.K.), JP18am0301010 (Y.K.), and JP18ae0101028 (Y.K.), and by JSPS KAKENHI Grant Number 17K07299 (M.K.K.), Grant Number 16K10748 (Y.K.), and Grant Number 19K07705 (Y.K.).
Funding Information:
Y.K. received research funding from ZENOAQ RESOURCE CO., LTD . The other authors have no conflict of interest.
Publisher Copyright:
© 2019 The Authors
PY - 2019/7
Y1 - 2019/7
N2 - Sensitive and specific monoclonal antibodies (mAbs)targeting podoplanin (PDPN)are needed for immunohistochemical analyses using formalin-fixed paraffin-embedded tissues because PDPN is known as a lymphatic endothelial cell maker in pathology. Recently, we established anti-PDPN mAbs against many species, such as human, mouse, rat, rabbit, dog, cat, bovine, pig, horse, goat, tiger, alpaca, and Tasmanian devil. However, anti-bear PDPN (bPDPN)has not been established yet. In this study, we immunized mice with bPDPN-overexpressing Chinese hamster ovary (CHO)-K1 (CHO/bPDPN)cells, and screened mAbs against bPDPN using flow cytometry. One of the mAbs, PMab-247 (IgG1, kappa), specifically detected CHO/bPDPN cells by flow cytometry and immunohistochemistry. Our findings suggest the potential usefulness of PMab-247 for the functional analyses of bPDPN.
AB - Sensitive and specific monoclonal antibodies (mAbs)targeting podoplanin (PDPN)are needed for immunohistochemical analyses using formalin-fixed paraffin-embedded tissues because PDPN is known as a lymphatic endothelial cell maker in pathology. Recently, we established anti-PDPN mAbs against many species, such as human, mouse, rat, rabbit, dog, cat, bovine, pig, horse, goat, tiger, alpaca, and Tasmanian devil. However, anti-bear PDPN (bPDPN)has not been established yet. In this study, we immunized mice with bPDPN-overexpressing Chinese hamster ovary (CHO)-K1 (CHO/bPDPN)cells, and screened mAbs against bPDPN using flow cytometry. One of the mAbs, PMab-247 (IgG1, kappa), specifically detected CHO/bPDPN cells by flow cytometry and immunohistochemistry. Our findings suggest the potential usefulness of PMab-247 for the functional analyses of bPDPN.
KW - Bear podoplanin
KW - Immunohistochemistry
KW - PDPN
KW - PMab-247
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U2 - 10.1016/j.bbrep.2019.100644
DO - 10.1016/j.bbrep.2019.100644
M3 - Article
AN - SCOPUS:85064640093
SN - 2405-5808
VL - 18
JO - Biochemistry and Biophysics Reports
JF - Biochemistry and Biophysics Reports
M1 - 100644
ER -