Development of azo-based fluorescent probes to detect different levels of hypoxia

Wen Piao, Satoru Tsuda, Yuji Tanaka, Satoshi Maeda, Fengyi Liu, Shodai Takahashi, Yu Kushida, Toru Komatsu, Tasuku Ueno, Takuya Terai, Toru Nakazawa, Masanobu Uchiyama, Keiji Morokuma, Tetsuo Nagano, Kenjiro Hanaoka

Research output: Contribution to journalArticlepeer-review

235 Citations (Scopus)


Let it shine: New hypoxia-sensitive fluorescent probes were developed; they consist of a rhodamine moiety with an azo group directly conjugated to the fluorophore. Because of an ultrafast conformational change around the N=N bond, the compounds are nonfluorescent under normoxia. However, under hypoxia, the azo group is reduced, and a strongly fluorescent rhodamine derivative is released.

Original languageEnglish
Pages (from-to)13028-13032
Number of pages5
JournalAngewandte Chemie - International Edition
Issue number49
Publication statusPublished - 2013 Dec 2


  • azo compounds
  • bioorganic chemistry
  • fluorescent probes
  • imaging agents
  • oxygen concentration


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