Bacterial DNA is expected to be a potent immune stimulating agent to Toll-like receptor (TLR) 9 expressed cells such as macrophages, monocytes, B lymphocytes, NK cells and dendritic cells. In the present study, we constructed a transfectant of swine TLR9 with mammalian cells. We demonstrated that the transfectant, recognizing both CpG and non-CpG oligonucleotides from lactic acid bacteria, induced NF-κB activation by gene reporter assay. These findings indicate that the swine TLR9 transfectant will be highly useful for the screening of immunostimulatory DNA from lactic acid bacteria.
- Lactic acid bacteria DNA
- Toll-like receptor 9