TY - JOUR
T1 - Development of mPMab-1, a Mouse-Rat Chimeric Antibody Against Mouse Podoplanin
AU - Yamada, Shinji
AU - Kaneko, Mika K.
AU - Nakamura, Takuro
AU - Ichii, Osamu
AU - Konnai, Satoru
AU - Kato, Yukinari
N1 - Publisher Copyright:
© 2017 Mary Ann Liebert, Inc.
PY - 2017/4
Y1 - 2017/4
N2 - Podoplanin (PDPN), the ligand of C-type lectin-like receptor-2, is used as a lymphatic endothelial marker. We previously established clone PMab-1 of rat IgG2a as a specific monoclonal antibody (mAb) against mouse PDPN. PMab-1 is also very sensitive in immunohistochemical analysis; however, rat mAbs seem to be unfavorable for pathologists because anti-mouse IgG and anti-rabbit IgG are usually used as secondary antibodies in commercially available kits for immunohistochemical analysis. In this study, we develop a mouse-rat chimeric antibody, mPMab-1 of mouse IgG2a, which was derived from rat PMab-1 mAb. Immunohistochemical analysis shows that mPMab-1 detects podocytes of the kidney, lymphatic endothelial cells of the colon, and type I alveolar cells of the lung. Importantly, mPMab-1 is more sensitive than PMab-1. This conversion strategy from rat mAb to mouse mAb could be applicable to other mAbs.
AB - Podoplanin (PDPN), the ligand of C-type lectin-like receptor-2, is used as a lymphatic endothelial marker. We previously established clone PMab-1 of rat IgG2a as a specific monoclonal antibody (mAb) against mouse PDPN. PMab-1 is also very sensitive in immunohistochemical analysis; however, rat mAbs seem to be unfavorable for pathologists because anti-mouse IgG and anti-rabbit IgG are usually used as secondary antibodies in commercially available kits for immunohistochemical analysis. In this study, we develop a mouse-rat chimeric antibody, mPMab-1 of mouse IgG2a, which was derived from rat PMab-1 mAb. Immunohistochemical analysis shows that mPMab-1 detects podocytes of the kidney, lymphatic endothelial cells of the colon, and type I alveolar cells of the lung. Importantly, mPMab-1 is more sensitive than PMab-1. This conversion strategy from rat mAb to mouse mAb could be applicable to other mAbs.
KW - chimeric antibody
KW - monoclonal antibody
KW - PMab-1
KW - podoplanin
UR - http://www.scopus.com/inward/record.url?scp=85018646590&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85018646590&partnerID=8YFLogxK
U2 - 10.1089/mab.2017.0002
DO - 10.1089/mab.2017.0002
M3 - Article
C2 - 28387612
AN - SCOPUS:85018646590
SN - 2167-9436
VL - 36
SP - 77
EP - 79
JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
IS - 2
ER -