Development of optical and acoustic hybrid microscope for visualization of a single cell

Joe Shikama; Yu Sato; Kazuto Kobayashi; Koki Yoshida; Ryo Nagaoka; Yoshifumi Saijo

doi:10.11239/jsmbe.52.O-345

Development of optical and acoustic hybrid microscope for visualization of a single cell

Joe Shikama, Yu Sato, Kazuto Kobayashi, Koki Yoshida, Ryo Nagaoka, Yoshifumi Saijo

MEN - Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

Acoustic microscope can measure the acoustic parameters of living cells. It is expected that disease mechanism is elucidated, and that treatment methods is developed by analyzing structure and function of cells. In this study, the optical and acoustic hybrid microscope has been developed. The ultrasonic transducer has 250 MHz. As a result of imaging the 3T3-L1 mouse fibroblasts, good contrast was achieved in the portion of the cell nucleus and pseudopod in the acoustic image. In addition, two-dimensional color images of thickness and sound speed of the cell were successfully obtained by using time-resolved analysis. The average thickness was 7.9 μm, and sound speed was 1602 m/s.

Original language	English
Pages (from-to)	O-345-O-346
Journal	Transactions of Japanese Society for Medical and Biological Engineering
Volume	52
DOIs	https://doi.org/10.11239/jsmbe.52.O-345
Publication status	Published - 2014 Aug 17

Keywords

Acoustic microscope
Ultrasonic hybrid microscope

ASJC Scopus subject areas

Biomedical Engineering

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10.11239/jsmbe.52.O-345

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abstract = "Acoustic microscope can measure the acoustic parameters of living cells. It is expected that disease mechanism is elucidated, and that treatment methods is developed by analyzing structure and function of cells. In this study, the optical and acoustic hybrid microscope has been developed. The ultrasonic transducer has 250 MHz. As a result of imaging the 3T3-L1 mouse fibroblasts, good contrast was achieved in the portion of the cell nucleus and pseudopod in the acoustic image. In addition, two-dimensional color images of thickness and sound speed of the cell were successfully obtained by using time-resolved analysis. The average thickness was 7.9 μm, and sound speed was 1602 m/s.",

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AU - Shikama, Joe

AU - Sato, Yu

AU - Kobayashi, Kazuto

AU - Yoshida, Koki

AU - Nagaoka, Ryo

AU - Saijo, Yoshifumi

PY - 2014/8/17

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AB - Acoustic microscope can measure the acoustic parameters of living cells. It is expected that disease mechanism is elucidated, and that treatment methods is developed by analyzing structure and function of cells. In this study, the optical and acoustic hybrid microscope has been developed. The ultrasonic transducer has 250 MHz. As a result of imaging the 3T3-L1 mouse fibroblasts, good contrast was achieved in the portion of the cell nucleus and pseudopod in the acoustic image. In addition, two-dimensional color images of thickness and sound speed of the cell were successfully obtained by using time-resolved analysis. The average thickness was 7.9 μm, and sound speed was 1602 m/s.

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Development of optical and acoustic hybrid microscope for visualization of a single cell

Abstract

Keywords

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