TY - JOUR
T1 - Development of Oxygen Consumption Analysis with an on-Chip Electrochemical Device and Simulation
AU - Hiramoto, Kaoru
AU - Yasumi, Masahiro
AU - Ushio, Hiroshi
AU - Shunori, Atsushi
AU - Ino, Kosuke
AU - Shiku, Hitoshi
AU - Matsue, Tomokazu
N1 - Funding Information:
The authors would like to thank T. Hisamoto, M. Yamamoto, and T. Hiratsuka for their aid in the development of the measurement system. We would also like to thank Prof. Hiroyuki Abe in Yamagata University for device development and Dr. N. Yaegashi and his team in Tohoku University Graduate School of Medicine for their assistance in the clinical study. This work was also supported in part by a Grant-in-Aid for Scientific Research (A) (No. 16H02280) and a Grant-in-Aid for Young Scientists (No. 15H05415) from the Japan Society for the Promotion of Science (JSPS).
Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/10/3
Y1 - 2017/10/3
N2 - The O2 consumption rate of embryos has been attracting much attention as a key indicator of cell metabolisms and development. In this study, we propose an on-chip device that enables the accurate, easy, and noninvasive measurement of O2 consumption rates of single embryos. Pt electrodes and micropits for embryo settlement were fabricated on Si chips via microfabrication techniques. The configuration of the device enables the detection of O2 concentration profiles surrounding the embryos by settling embryos into the pits with a mouth pipet. Moreover, as the detection is based on an electrochemical method, the influence of O2 consumption on the electrodes was also considered. By using a simulator (COMSOL Multiphysics), we estimated the O2 concentration profiles in the device with and without the effects of the electrodes. Based on the simulation results, we developed a normalization process to calculate the precise O2 consumption rate of the sample. Finally, using both the measurement system and the algorithm for the analysis, the respiratory activities of mouse embryos were successfully measured.
AB - The O2 consumption rate of embryos has been attracting much attention as a key indicator of cell metabolisms and development. In this study, we propose an on-chip device that enables the accurate, easy, and noninvasive measurement of O2 consumption rates of single embryos. Pt electrodes and micropits for embryo settlement were fabricated on Si chips via microfabrication techniques. The configuration of the device enables the detection of O2 concentration profiles surrounding the embryos by settling embryos into the pits with a mouth pipet. Moreover, as the detection is based on an electrochemical method, the influence of O2 consumption on the electrodes was also considered. By using a simulator (COMSOL Multiphysics), we estimated the O2 concentration profiles in the device with and without the effects of the electrodes. Based on the simulation results, we developed a normalization process to calculate the precise O2 consumption rate of the sample. Finally, using both the measurement system and the algorithm for the analysis, the respiratory activities of mouse embryos were successfully measured.
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U2 - 10.1021/acs.analchem.7b02074
DO - 10.1021/acs.analchem.7b02074
M3 - Article
C2 - 28876053
AN - SCOPUS:85030681673
SN - 0003-2700
VL - 89
SP - 10303
EP - 10310
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 19
ER -