TY - JOUR
T1 - Development of peptide arrays for detection of IgE-binding epitopes in cow's milk allergens
AU - Matsumoto, Naoki
AU - Okochi, Mina
AU - Matsushima, Miyoko
AU - Ogawa, Akiko
AU - Takase, Tomokazu
AU - Yoshida, Yasuko
AU - Kawase, Mitsuo
AU - Isobe, Ken ichi
AU - Kawabe, Tsutomu
AU - Honda, Hiroyuki
N1 - Funding Information:
The authors would like to thank Associate Professor Tatsuo Sakamoto from Nagoya University Hospital, and Dr. Koumei Ito from Aichi Children's Health and Medical Center, Aichi, Japan, for kindly providing us the sera samples and discussion. This study was performed through Special Coordination Funds for Promoting Science and Technology of the Ministry of Education, Culture, Sports, Science and Technology, the Japanese Government.
PY - 2009/3
Y1 - 2009/3
N2 - Peptide arrays have become versatile tools for high throughput screening assays in biomedical and pharmaceutical research. In this study, we constructed a peptide array that contained linear peptide fragments reported as IgE-binding epitopes for cow's milk allergy (CMA). Various peptides with different solubility in aqueous solutions were dissolved in the buffer solutions containing sodium dodecyl sulfate, and we achieved a consistent spotting of peptide solutions using a piezoelectric ceramic micropump. The IgE-binding patterns were successfully detected by observing the binding of Alexa 647-labeled anti-human IgE using sera from CMA patients. Our technique in this study will provide a potent capability for the development of a peptide array for mapping IgE-epitopes in milk proteins, and it will help researchers better understand the IgE-epitopes associated with the clinical outcome of CMA.
AB - Peptide arrays have become versatile tools for high throughput screening assays in biomedical and pharmaceutical research. In this study, we constructed a peptide array that contained linear peptide fragments reported as IgE-binding epitopes for cow's milk allergy (CMA). Various peptides with different solubility in aqueous solutions were dissolved in the buffer solutions containing sodium dodecyl sulfate, and we achieved a consistent spotting of peptide solutions using a piezoelectric ceramic micropump. The IgE-binding patterns were successfully detected by observing the binding of Alexa 647-labeled anti-human IgE using sera from CMA patients. Our technique in this study will provide a potent capability for the development of a peptide array for mapping IgE-epitopes in milk proteins, and it will help researchers better understand the IgE-epitopes associated with the clinical outcome of CMA.
KW - Cow's milk allergy
KW - IgE-binding epitopes
KW - Peptide array
KW - Piezoelectric ceramic micropump
KW - spotting buffer
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U2 - 10.1016/j.jbiosc.2008.10.014
DO - 10.1016/j.jbiosc.2008.10.014
M3 - Article
C2 - 19269601
AN - SCOPUS:61349191101
SN - 1389-1723
VL - 107
SP - 324
EP - 330
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 3
ER -