@article{75ae3667c99a46859455fac1548d1950,
title = "Development of RAP Tag, a Novel Tagging System for Protein Detection and Purification",
abstract = "Affinity tag systems, possessing high affinity and specificity, are useful for protein detection and purification. The most suitable tag for a particular purpose should be selected from many available affinity tag systems. In this study, we developed a novel affinity tag called the {"}RAP tag{"} system, which comprises a mouse antirat podoplanin monoclonal antibody (clone PMab-2) and the RAP tag (DMVNPGLEDRIE). This system is useful not only for protein detection in Western blotting, flow cytometry, and sandwich enzyme-linked immunosorbent assay, but also for protein purification.",
keywords = "affinity tag, monoclonal antibody, podoplanin, protein purification, RAP tag",
author = "Yuki Fujii and Kaneko, {Mika K.} and Satoshi Ogasawara and Shinji Yamada and Miyuki Yanaka and Takuro Nakamura and Noriko Saidoh and Kanae Yoshida and Ryusuke Honma and Yukinari Kato",
note = "Funding Information: This work was supported mainly by the Basic Science and Platform Technology Program for Innovative Biological Medicine from Japan Agency for Medical Research and Development, AMED (Y.K.). This work was also supported in part by the Platform for Drug Discovery, Informatics, and Structural Life Science (PDIS) from AMED (Y.K.), by Project for utilizing glycans in the development of innovative drug discovery technologies from AMED (Y.K.), by Translational Research Network Program from AMED (Y.K.), by the Regional Innovation Strategy Support Program from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan (Y.K.), by JSPS KAKENHI Grant Numbers 26440019 (M.K.K.) and 16K10748 (Y.K.). Publisher Copyright: {\textcopyright} 2017 Yuki Fujii et al.",
year = "2017",
month = apr,
doi = "10.1089/mab.2016.0052",
language = "English",
volume = "36",
pages = "68--71",
journal = "Monoclonal Antibodies in Immunodiagnosis and Immunotherapy",
issn = "2167-9436",
publisher = "Mary Ann Liebert Inc.",
number = "2",
}