Affinity tag systems, possessing high affinity and specificity, are useful for protein detection and purification. The most suitable tag for a particular purpose should be selected from many available affinity tag systems. In this study, we developed a novel affinity tag called the "RAP tag" system, which comprises a mouse antirat podoplanin monoclonal antibody (clone PMab-2) and the RAP tag (DMVNPGLEDRIE). This system is useful not only for protein detection in Western blotting, flow cytometry, and sandwich enzyme-linked immunosorbent assay, but also for protein purification.
|Number of pages||4|
|Journal||Monoclonal Antibodies in Immunodiagnosis and Immunotherapy|
|Publication status||Published - 2017 Apr|
- affinity tag
- monoclonal antibody
- protein purification
- RAP tag