Developmental changes of gene expression in heme metabolic enzymes in rat placenta

Naomi Ihara; Reiko Akagi; Kohei Ejiri; Takafumi Kudo; Kazumichi Furuyama; Hiroyoshi Fujita

doi:10.1016/S0014-5793(98)01324-6

Developmental changes of gene expression in heme metabolic enzymes in rat placenta

Naomi Ihara, Reiko Akagi, Kohei Ejiri, Takafumi Kudo, Kazumichi Furuyama, Hiroyoshi Fujita

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Transcription levels of the non-specific δ-aminolevulinate synthase (ALAS-N) and heme oxygenase-1 (HO-1) in the placenta at the terminal stage of pregnancy were comparable to those in the female adult liver and in the spleen, respectively. Immunohistochemical studies demonstrated that both enzymes were exclusively expressed in the trophoblast. During gestation, transcript of ALAS-N slightly increased, while HO-1 mRNA significantly decreased. Induced acute fetal hypoxia resulted in an increase in ALAS-N mRNA and in a decrease in HO-1 mRNA. These findings indicate that placental heme metabolism is influenced by the oxygen supply. Copyright (C) 1998 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	163-167
Number of pages	5
Journal	FEBS Letters
Volume	439
Issue number	1-2
DOIs	https://doi.org/10.1016/S0014-5793(98)01324-6
Publication status	Published - 1998 Nov 13
Externally published	Yes

Keywords

Fetus
Heme
Heme oxygenase
Hypoxia
Placenta
Trophoblast
δ-Aminolevulinate synthase

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Developmental changes of gene expression in heme metabolic enzymes in rat placenta",

abstract = "Transcription levels of the non-specific δ-aminolevulinate synthase (ALAS-N) and heme oxygenase-1 (HO-1) in the placenta at the terminal stage of pregnancy were comparable to those in the female adult liver and in the spleen, respectively. Immunohistochemical studies demonstrated that both enzymes were exclusively expressed in the trophoblast. During gestation, transcript of ALAS-N slightly increased, while HO-1 mRNA significantly decreased. Induced acute fetal hypoxia resulted in an increase in ALAS-N mRNA and in a decrease in HO-1 mRNA. These findings indicate that placental heme metabolism is influenced by the oxygen supply. Copyright (C) 1998 Federation of European Biochemical Societies.",

keywords = "Fetus, Heme, Heme oxygenase, Hypoxia, Placenta, Trophoblast, δ-Aminolevulinate synthase",

author = "Naomi Ihara and Reiko Akagi and Kohei Ejiri and Takafumi Kudo and Kazumichi Furuyama and Hiroyoshi Fujita",

note = "Funding Information: We are grateful to Dr. S. Shibahara (Tohoku University, Sendai, Japan) and Dr. M. Yamamoto (Tsukuba University, Tsukuba, Japan) for providing us with pRHO-1 and pKRA2cA and anti-rat liver ALAS antibody, respectively. We are also grateful to Dr. S. Sassa (The Rockefeller University, New York, USA) for his helpful suggestions and manuscript reviewing. This study was supported in part by a grant-in-aid for scientific research from the Ministry of Education, Science and Culture of Japan. (to R.A., T.K. and H.F.).",

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doi = "10.1016/S0014-5793(98)01324-6",

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AU - Ihara, Naomi

AU - Akagi, Reiko

AU - Ejiri, Kohei

AU - Kudo, Takafumi

AU - Furuyama, Kazumichi

AU - Fujita, Hiroyoshi

N1 - Funding Information: We are grateful to Dr. S. Shibahara (Tohoku University, Sendai, Japan) and Dr. M. Yamamoto (Tsukuba University, Tsukuba, Japan) for providing us with pRHO-1 and pKRA2cA and anti-rat liver ALAS antibody, respectively. We are also grateful to Dr. S. Sassa (The Rockefeller University, New York, USA) for his helpful suggestions and manuscript reviewing. This study was supported in part by a grant-in-aid for scientific research from the Ministry of Education, Science and Culture of Japan. (to R.A., T.K. and H.F.).

PY - 1998/11/13

Y1 - 1998/11/13

N2 - Transcription levels of the non-specific δ-aminolevulinate synthase (ALAS-N) and heme oxygenase-1 (HO-1) in the placenta at the terminal stage of pregnancy were comparable to those in the female adult liver and in the spleen, respectively. Immunohistochemical studies demonstrated that both enzymes were exclusively expressed in the trophoblast. During gestation, transcript of ALAS-N slightly increased, while HO-1 mRNA significantly decreased. Induced acute fetal hypoxia resulted in an increase in ALAS-N mRNA and in a decrease in HO-1 mRNA. These findings indicate that placental heme metabolism is influenced by the oxygen supply. Copyright (C) 1998 Federation of European Biochemical Societies.

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KW - δ-Aminolevulinate synthase

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Developmental changes of gene expression in heme metabolic enzymes in rat placenta

Abstract

Keywords

ASJC Scopus subject areas

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