Dielectrophoretic micropatterning with Microparticle monolayers covalently linked to glass surfaces

Masato Suzuki, Tomoyuki Yasukawa, Yoshiaki Mase, Daisuke Oyamatsu, Hitoshi Shiku, Tomokazu Matsue

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92 Citations (Scopus)


Two-dimensional micropatterns of microparticles were fabricated on glass substrates with negative dielectrophoretic force, and the patterned microparticles were covalently bound on the substrate via cross-linking agents. The line and grid patterns of microparticles were prepared using the repulsive force of negative dielectrophoresis (n-DEP). The template interdigitated microband array (IDA) electrodes (width and gap 50 μm) were incorporated into the dielectrophoretic patterning cell with a fluidic channel. The microstructures on the glass substrates with amino or sulfhydryl groups were immobilized with the cross-linking agents disuccinimidyl suberate (D3S) and m-maleimidobenzoyl-N-hydroxy-succinimide ester (MBS). Diaphorase (Dp), a flavoenzyme, was selectively attached on the patterned microparticles using the maleimide groups of MBS. The enzyme activity on the patterned particles was electrochemically characterized with a scanning electrochemical microscope (SECM) in the presence of NADH and ferrocenylmethanol as a redox mediator. The SECM images proved that Dp was selectively immobilized onto the surface of microparticles to maintain its catalytic activity.

Original languageEnglish
Pages (from-to)11005-11011
Number of pages7
Issue number25
Publication statusPublished - 2004 Dec 7


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