Dietary administration of tetrodotoxin and its putative biosynthetic intermediates to the captive-reared non-toxic Japanese fire-bellied newt, Cynops pyrrhogaster

Yuta Kudo; Chikafumi Chiba; Keiichi Konoki; Yuko Cho; Mari Yotsu-Yamashita

doi:10.1016/j.toxicon.2017.07.016

Dietary administration of tetrodotoxin and its putative biosynthetic intermediates to the captive-reared non-toxic Japanese fire-bellied newt, Cynops pyrrhogaster

Yuta Kudo, Chikafumi Chiba, Keiichi Konoki, Yuko Cho, Mari Yotsu-Yamashita

University of Tsukuba

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The origin of tetrodotoxin (TTX) in amphibians has long been disputed. In this study, TTX and its putative biosynthetic intermediates or shunt compounds (4,9-anhydro-10-hemiketal-5-deoxyTTX and Cep-242) were dietary administered to the captive-reared, non-toxic Japanese fire-bellied newt, Cynops pyrrhogaster. After 4 weeks, the ingested compounds were detected mainly in the newt body using liquid chromatography-mass spectrometry (LC-MS), while these compounds were not converted into other TTX analogues in newts.

Original language	English
Pages (from-to)	78-82
Number of pages	5
Journal	Toxicon
Volume	137
DOIs	https://doi.org/10.1016/j.toxicon.2017.07.016
Publication status	Published - 2017 Oct

Keywords

Cynops pyrrhogaster
LC-MS
Newt
Tetrodotoxin
Toxin administration

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10.1016/j.toxicon.2017.07.016

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title = "Dietary administration of tetrodotoxin and its putative biosynthetic intermediates to the captive-reared non-toxic Japanese fire-bellied newt, Cynops pyrrhogaster",

abstract = "The origin of tetrodotoxin (TTX) in amphibians has long been disputed. In this study, TTX and its putative biosynthetic intermediates or shunt compounds (4,9-anhydro-10-hemiketal-5-deoxyTTX and Cep-242) were dietary administered to the captive-reared, non-toxic Japanese fire-bellied newt, Cynops pyrrhogaster. After 4 weeks, the ingested compounds were detected mainly in the newt body using liquid chromatography-mass spectrometry (LC-MS), while these compounds were not converted into other TTX analogues in newts.",

keywords = "Cynops pyrrhogaster, LC-MS, Newt, Tetrodotoxin, Toxin administration",

author = "Yuta Kudo and Chikafumi Chiba and Keiichi Konoki and Yuko Cho and Mari Yotsu-Yamashita",

note = "Funding Information: This work was funded by Japan Society for the Promotion of Science (JSPS) through its Funding Program for the Next Generation World-Leading Researchers (LS012) and KAKENHI Grant-in-Aid for Scientific Research (B) no. 26292057 and 17H03809 to M.Y.Y. Y.K. was a research fellow of JSPS (DC1) (no. 25-5534). We thank Dr. Clyde Gorapava Puilingi for assistance in the maintaining the newts. Publisher Copyright: {\textcopyright} 2017 Elsevier Ltd",

year = "2017",

month = oct,

doi = "10.1016/j.toxicon.2017.07.016",

language = "English",

volume = "137",

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AU - Kudo, Yuta

AU - Chiba, Chikafumi

AU - Konoki, Keiichi

AU - Cho, Yuko

AU - Yotsu-Yamashita, Mari

N1 - Funding Information: This work was funded by Japan Society for the Promotion of Science (JSPS) through its Funding Program for the Next Generation World-Leading Researchers (LS012) and KAKENHI Grant-in-Aid for Scientific Research (B) no. 26292057 and 17H03809 to M.Y.Y. Y.K. was a research fellow of JSPS (DC1) (no. 25-5534). We thank Dr. Clyde Gorapava Puilingi for assistance in the maintaining the newts. Publisher Copyright: © 2017 Elsevier Ltd

PY - 2017/10

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N2 - The origin of tetrodotoxin (TTX) in amphibians has long been disputed. In this study, TTX and its putative biosynthetic intermediates or shunt compounds (4,9-anhydro-10-hemiketal-5-deoxyTTX and Cep-242) were dietary administered to the captive-reared, non-toxic Japanese fire-bellied newt, Cynops pyrrhogaster. After 4 weeks, the ingested compounds were detected mainly in the newt body using liquid chromatography-mass spectrometry (LC-MS), while these compounds were not converted into other TTX analogues in newts.

AB - The origin of tetrodotoxin (TTX) in amphibians has long been disputed. In this study, TTX and its putative biosynthetic intermediates or shunt compounds (4,9-anhydro-10-hemiketal-5-deoxyTTX and Cep-242) were dietary administered to the captive-reared, non-toxic Japanese fire-bellied newt, Cynops pyrrhogaster. After 4 weeks, the ingested compounds were detected mainly in the newt body using liquid chromatography-mass spectrometry (LC-MS), while these compounds were not converted into other TTX analogues in newts.

KW - Cynops pyrrhogaster

KW - LC-MS

KW - Newt

KW - Tetrodotoxin

KW - Toxin administration

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Dietary administration of tetrodotoxin and its putative biosynthetic intermediates to the captive-reared non-toxic Japanese fire-bellied newt, Cynops pyrrhogaster

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Keywords

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