Differential expression of two glucuronyltransferases synthesizing HNK-1 carbohydrate epitope in the sublineages of the rat myogenic progenitors

Takashi Nagase; Yasushi Shimoda; Yutaka Sanai; Shun Nakamura; Kiyonori Harii; Noriko Osumi

doi:10.1016/S0925-4773(00)00449-4

Differential expression of two glucuronyltransferases synthesizing HNK-1 carbohydrate epitope in the sublineages of the rat myogenic progenitors

Takashi Nagase, Yasushi Shimoda, Yutaka Sanai, Shun Nakamura, Kiyonori Harii, Noriko Osumi

MED - United Centers for Advanced Research and Translational Medicine (ART)

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

HNK-1 epitope is a cell-surface carbohydrate mediating various cell-cell or cell-substrate interactions. We found HNK-1 epitope in longitudinally arrayed fibers in the subpopulation of the epaxial myotome, and hypaxial myoblasts migrating into the limb bud in the rat embryo. We next investigated the expression patterns of genes encoding two glucuronyltransferases (GlcAT-P, GlcAT-D) and sulfotransferase (Sul-T), which are required for biosynthesis of HNK-1 epitope. GlcAT-P gene was expressed in the non-migrating longitudinal fibers, whereas GlcAT-D gene was expressed in the migrating myoblasts in the limb bud. Sul-T gene expression was ubiquitously observed in all these myogenic populations. Thus, differential expression of GlcAT genes may relate to the epaxial/hypaxial or migrating/non-migrating myoblast lineages. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original language	English
Pages (from-to)	145-149
Number of pages	5
Journal	Mechanisms of Development
Volume	98
Issue number	1-2
DOIs	https://doi.org/10.1016/S0925-4773(00)00449-4
Publication status	Published - 2000 Nov 1

Keywords

Epaxial myoblast
GlcAT-D
GlcAT-P
Glucuronyltransferase
HNK-1 epitope
Hypaxial myoblast
Migration
Myotome
Sulfotransferase

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10.1016/S0925-4773(00)00449-4

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title = "Differential expression of two glucuronyltransferases synthesizing HNK-1 carbohydrate epitope in the sublineages of the rat myogenic progenitors",

abstract = "HNK-1 epitope is a cell-surface carbohydrate mediating various cell-cell or cell-substrate interactions. We found HNK-1 epitope in longitudinally arrayed fibers in the subpopulation of the epaxial myotome, and hypaxial myoblasts migrating into the limb bud in the rat embryo. We next investigated the expression patterns of genes encoding two glucuronyltransferases (GlcAT-P, GlcAT-D) and sulfotransferase (Sul-T), which are required for biosynthesis of HNK-1 epitope. GlcAT-P gene was expressed in the non-migrating longitudinal fibers, whereas GlcAT-D gene was expressed in the migrating myoblasts in the limb bud. Sul-T gene expression was ubiquitously observed in all these myogenic populations. Thus, differential expression of GlcAT genes may relate to the epaxial/hypaxial or migrating/non-migrating myoblast lineages. Copyright (C) 2000 Elsevier Science Ireland Ltd.",

keywords = "Epaxial myoblast, GlcAT-D, GlcAT-P, Glucuronyltransferase, HNK-1 epitope, Hypaxial myoblast, Migration, Myotome, Sulfotransferase",

author = "Takashi Nagase and Yasushi Shimoda and Yutaka Sanai and Shun Nakamura and Kiyonori Harii and Noriko Osumi",

note = "Funding Information: We thank T. Inoue, Y. Ishii, N. Takashima, Y. Wakamatsu and all the members of the Shindan laboratory for discussions and helpful suggestions, and J. Asami for technical assistance. This work was supported by a Grant-in Aid for Scientific Research from the Japanese Ministry of Education, Science and Culture (Nos. 1022029, 11470002 and 1158090), CREST from Japanese Science and Technology Corporation, and Human Frontier Science Program Organization (RG0145/1998-B) given to N.O. T.N. is the recipient of a Fellowship from the Japan Society for Promotion of Science.",

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doi = "10.1016/S0925-4773(00)00449-4",

language = "English",

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TY - JOUR

T1 - Differential expression of two glucuronyltransferases synthesizing HNK-1 carbohydrate epitope in the sublineages of the rat myogenic progenitors

AU - Nagase, Takashi

AU - Shimoda, Yasushi

AU - Sanai, Yutaka

AU - Nakamura, Shun

AU - Harii, Kiyonori

AU - Osumi, Noriko

N1 - Funding Information: We thank T. Inoue, Y. Ishii, N. Takashima, Y. Wakamatsu and all the members of the Shindan laboratory for discussions and helpful suggestions, and J. Asami for technical assistance. This work was supported by a Grant-in Aid for Scientific Research from the Japanese Ministry of Education, Science and Culture (Nos. 1022029, 11470002 and 1158090), CREST from Japanese Science and Technology Corporation, and Human Frontier Science Program Organization (RG0145/1998-B) given to N.O. T.N. is the recipient of a Fellowship from the Japan Society for Promotion of Science.

PY - 2000/11/1

Y1 - 2000/11/1

N2 - HNK-1 epitope is a cell-surface carbohydrate mediating various cell-cell or cell-substrate interactions. We found HNK-1 epitope in longitudinally arrayed fibers in the subpopulation of the epaxial myotome, and hypaxial myoblasts migrating into the limb bud in the rat embryo. We next investigated the expression patterns of genes encoding two glucuronyltransferases (GlcAT-P, GlcAT-D) and sulfotransferase (Sul-T), which are required for biosynthesis of HNK-1 epitope. GlcAT-P gene was expressed in the non-migrating longitudinal fibers, whereas GlcAT-D gene was expressed in the migrating myoblasts in the limb bud. Sul-T gene expression was ubiquitously observed in all these myogenic populations. Thus, differential expression of GlcAT genes may relate to the epaxial/hypaxial or migrating/non-migrating myoblast lineages. Copyright (C) 2000 Elsevier Science Ireland Ltd.

AB - HNK-1 epitope is a cell-surface carbohydrate mediating various cell-cell or cell-substrate interactions. We found HNK-1 epitope in longitudinally arrayed fibers in the subpopulation of the epaxial myotome, and hypaxial myoblasts migrating into the limb bud in the rat embryo. We next investigated the expression patterns of genes encoding two glucuronyltransferases (GlcAT-P, GlcAT-D) and sulfotransferase (Sul-T), which are required for biosynthesis of HNK-1 epitope. GlcAT-P gene was expressed in the non-migrating longitudinal fibers, whereas GlcAT-D gene was expressed in the migrating myoblasts in the limb bud. Sul-T gene expression was ubiquitously observed in all these myogenic populations. Thus, differential expression of GlcAT genes may relate to the epaxial/hypaxial or migrating/non-migrating myoblast lineages. Copyright (C) 2000 Elsevier Science Ireland Ltd.

KW - Epaxial myoblast

KW - GlcAT-D

KW - GlcAT-P

KW - Glucuronyltransferase

KW - HNK-1 epitope

KW - Hypaxial myoblast

KW - Migration

KW - Myotome

KW - Sulfotransferase

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DO - 10.1016/S0925-4773(00)00449-4

M3 - Article

C2 - 11044619

AN - SCOPUS:0034333487

SN - 0925-4773

VL - 98

SP - 145

EP - 149

JO - Mechanisms of Development

JF - Mechanisms of Development

IS - 1-2

ER -

Differential expression of two glucuronyltransferases synthesizing HNK-1 carbohydrate epitope in the sublineages of the rat myogenic progenitors

Abstract

Keywords

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