TY - JOUR
T1 - Differential regulation of diacylglycerol kinase isozymes in cardiac hypertrophy
AU - Yahagi, Hirokazu
AU - Takeda, Morihiko
AU - Asaumi, Yasuhide
AU - Okumura, Kenji
AU - Takahashi, Ryotaro
AU - Takahashi, Jun
AU - Ohta, Jun
AU - Tada, Hiroko
AU - Minatoya, Yutaka
AU - Sakuma, Masahito
AU - Watanabe, Jun
AU - Goto, Kaoru
AU - Shirato, Kunio
AU - Kagaya, Yutaka
N1 - Funding Information:
This work was supported by Grants-in-Aid (15590715) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. We thank Dr. Ryoichi Yamaji (Division of Applied Biological Chemistry, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Osaka, Japan) for kindly providing anti-TPI antibody. We also thank Mr. Brent Bell for reading the manuscript.
PY - 2005/6/24
Y1 - 2005/6/24
N2 - To examine the involvement of diacylglycerol kinase (DGK) and phosphatidic acid phosphatase (PAP) in pressure overloaded cardiac hypertrophy, rats were subjected to either ascending aortic banding for 3, 7, and 28 days or sham operation. In comparison with sham-operated rats, the left ventricular (LV) weight of the aortic-banded rats increased progressively. At 28 days after surgery, the expression of DGKε mRNA but not DGKζ or PAP2b mRNA in the LV myocardium significantly decreased in the aortic-banded rats compared with the sham-operated rats. DGKζ protein in the LV myocardium translocated from the particulate to the cytosolic compartment in the aortic-banded rats. Furthermore, the myocardial content of 1,2-diacylglycerol and PKCδ protein expression in the particulate fraction of the LV myocardium significantly increased in aortic-banded rats compared with sham-operated rats. These results suggest that DGKε and DGKζ play distinct roles in the development of pressure overloaded cardiac hypertrophy and that the two isozymes are differentially regulated.
AB - To examine the involvement of diacylglycerol kinase (DGK) and phosphatidic acid phosphatase (PAP) in pressure overloaded cardiac hypertrophy, rats were subjected to either ascending aortic banding for 3, 7, and 28 days or sham operation. In comparison with sham-operated rats, the left ventricular (LV) weight of the aortic-banded rats increased progressively. At 28 days after surgery, the expression of DGKε mRNA but not DGKζ or PAP2b mRNA in the LV myocardium significantly decreased in the aortic-banded rats compared with the sham-operated rats. DGKζ protein in the LV myocardium translocated from the particulate to the cytosolic compartment in the aortic-banded rats. Furthermore, the myocardial content of 1,2-diacylglycerol and PKCδ protein expression in the particulate fraction of the LV myocardium significantly increased in aortic-banded rats compared with sham-operated rats. These results suggest that DGKε and DGKζ play distinct roles in the development of pressure overloaded cardiac hypertrophy and that the two isozymes are differentially regulated.
KW - 1,2-Diacylglycerol
KW - Cardiac hypertrophy
KW - Diacylglycerol kinase
KW - Intracellular translocation
KW - Phosphatidic acid phosphatase
KW - Protein kinase C
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U2 - 10.1016/j.bbrc.2005.04.094
DO - 10.1016/j.bbrc.2005.04.094
M3 - Article
C2 - 15896305
AN - SCOPUS:21144450627
SN - 0006-291X
VL - 332
SP - 101
EP - 108
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -