TY - JOUR
T1 - Differentiation and monitoring of cells using a biochip for regenerative medicine. (Differentiation of bone marrow stromal cells and myoblasts)
AU - Uchida, Tomoyuki
AU - Arai, Fumihito
AU - Suzuki, Osamu
AU - Ichikawa, Akihiko
AU - Fukuda, Toshio
AU - Katagiri, Takenobu
AU - Kamijo, Ryutaro
AU - Nakamura, Masanori
AU - Numata, Mamoru
AU - Watanabe, Naruaki
PY - 2007/3/15
Y1 - 2007/3/15
N2 - A novel biochip is developed for culturing stem cells. Biochip is made of Polymer (PDMS), and cells can be loaded by gradient strains in one chip. They grow well on a hydrophilic membrane and differentiation is promoted by cyclic strains. In this paper, we propose the method for culturing and monitoring of stem cells such as bone marrow stromal cells (ST2 cells) and myoblasts (C2C12 cells), and the results of culture. First we analyzed strains on a membrane when an air hole is decompressed, and clarified their range. From experiment, bone marrow stromal cells grew well in a narrow range, and we quantified their ALP activity as a measure of differentiation. As myoblasts, the direction of their differentiation was perpendicular to a groove, that is, the same direction of uniaxial strains.
AB - A novel biochip is developed for culturing stem cells. Biochip is made of Polymer (PDMS), and cells can be loaded by gradient strains in one chip. They grow well on a hydrophilic membrane and differentiation is promoted by cyclic strains. In this paper, we propose the method for culturing and monitoring of stem cells such as bone marrow stromal cells (ST2 cells) and myoblasts (C2C12 cells), and the results of culture. First we analyzed strains on a membrane when an air hole is decompressed, and clarified their range. From experiment, bone marrow stromal cells grew well in a narrow range, and we quantified their ALP activity as a measure of differentiation. As myoblasts, the direction of their differentiation was perpendicular to a groove, that is, the same direction of uniaxial strains.
KW - Biological engineering
KW - Medical engineering
KW - Micromachine
KW - Muscle and skeleton
KW - Regenerative medicine
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U2 - 10.1299/jsmec.49.852
DO - 10.1299/jsmec.49.852
M3 - Article
AN - SCOPUS:34147163037
SN - 1344-7653
VL - 49
SP - 852
EP - 858
JO - JSME International Journal, Series C: Mechanical Systems, Machine Elements and Manufacturing
JF - JSME International Journal, Series C: Mechanical Systems, Machine Elements and Manufacturing
IS - 3
ER -