TY - JOUR
T1 - Differentiation and monitoring of cells using a biochip for regenerative medicine (differentiation of bone marrow stromal cells and myoblasts)
AU - Arai, Fumihito
AU - Suzuki, Osamu
AU - Uchida, Tomoyuki
AU - Ichikawa, Akihiko
AU - Fukuda, Toshio
AU - Katagiri, Takenobu
AU - Kamijo, Ryutaro
AU - Nakamura, Masanori
AU - Numata, Mamoru
AU - Watanabe, Naruaki
PY - 2005/11
Y1 - 2005/11
N2 - A novel biochip is developed for culturing stem cells. Biochip is made of Polymer (PDMS), and cells can be loaded by gradient strains in one chip. They grow well on a hydrophilic membrane and differentiation is promoted by cyclic strains. In this paper, we propose the method for culturing and monitoring of stem cells such as bone marrow stromal cells (ST 2 cells) and myoblasts (C 2 C 12 cells), and the results of culture. First we analyzed strains on a membrane when an air hole is decompressed, and clarified their range. From experiment, bone marrow stromal cells grew well in a narrow range, and we quantified their ALP activity as a measure of differentiation. As myoblasts, the direction of their differentiation was perpendicular to a groove, that is, the same direction of uniaxial strains.
AB - A novel biochip is developed for culturing stem cells. Biochip is made of Polymer (PDMS), and cells can be loaded by gradient strains in one chip. They grow well on a hydrophilic membrane and differentiation is promoted by cyclic strains. In this paper, we propose the method for culturing and monitoring of stem cells such as bone marrow stromal cells (ST 2 cells) and myoblasts (C 2 C 12 cells), and the results of culture. First we analyzed strains on a membrane when an air hole is decompressed, and clarified their range. From experiment, bone marrow stromal cells grew well in a narrow range, and we quantified their ALP activity as a measure of differentiation. As myoblasts, the direction of their differentiation was perpendicular to a groove, that is, the same direction of uniaxial strains.
KW - Biological Engineering
KW - Medical Engineering
KW - Micromachine
KW - Muscle and Skelton
KW - Regenerative Medicine
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UR - http://www.scopus.com/inward/citedby.url?scp=33344474710&partnerID=8YFLogxK
U2 - 10.1299/kikaic.71.3239
DO - 10.1299/kikaic.71.3239
M3 - Review article
AN - SCOPUS:33344474710
SN - 0387-5024
VL - 71
SP - 3239
EP - 3245
JO - Nihon Kikai Gakkai Ronbunshu, C Hen/Transactions of the Japan Society of Mechanical Engineers, Part C
JF - Nihon Kikai Gakkai Ronbunshu, C Hen/Transactions of the Japan Society of Mechanical Engineers, Part C
IS - 11
ER -