Differentiation-dependent enhanced expression of protein phosphatase 2Cβ in germ cells of mouse seminiferous tubules

Shunsuke Kato; Takayasu Kobayashi; Kazuyuki Kusuda; Yukio Nishina; Yoshitake Nishimune; Kentaro Yomogida; Masayuki Yamamoto; Hiroyuki Sakagami; Hisatake Kondo; Motoko Ohnishi; Naoki Chida; Yuchio Yanagawa; Shinri Tamura

doi:10.1016/0014-5793(96)01119-2

Differentiation-dependent enhanced expression of protein phosphatase 2Cβ in germ cells of mouse seminiferous tubules

Shunsuke Kato, Takayasu Kobayashi, Kazuyuki Kusuda, Yukio Nishina, Yoshitake Nishimune, Kentaro Yomogida, Masayuki Yamamoto, Hiroyuki Sakagami, Hisatake Kondo, Motoko Ohnishi, Naoki Chida, Yuchio Yanagawa, Shinri Tamura

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Abstract

The presence of five distinct isoforms of protein phosphatase 2Cβ (PP2Cβ-1 ~ -5) is known. In this study, we demonstrate that the mRNA levels of PP2Cβ-3, -4 and -5 and PP2Cβ protein level increased during the course of the first wave of spermatogenesis in neonatal mouse testis. Northern blot and in situ hybridization analyses revealed that PP2Cβ-3, -4 and -5 were expressed predominantly in pachytene spermatocytes and in more highly differentiated germ cells. The substrate specificity of PP2Cβ-4 determined with artificial substrates differed from those of PP2Cβ-3 and -5, suggesting that the difference in the structure of PP2Cβ-3, -4 and -5 reflect their unique physiological functions in testicular germ cells.

Original language	English
Pages (from-to)	293-297
Number of pages	5
Journal	FEBS Letters
Volume	396
Issue number	2-3
DOIs	https://doi.org/10.1016/0014-5793(96)01119-2
Publication status	Published - 1996 Nov 4

Keywords

Isoform
Protein phosphatase 2C
Spermatogenesis
Testicular germ cell

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10.1016/0014-5793(96)01119-2

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Kato, S., Kobayashi, T., Kusuda, K., Nishina, Y., Nishimune, Y., Yomogida, K., Yamamoto, M., Sakagami, H., Kondo, H., Ohnishi, M., Chida, N., Yanagawa, Y., & Tamura, S. (1996). Differentiation-dependent enhanced expression of protein phosphatase 2Cβ in germ cells of mouse seminiferous tubules. FEBS Letters, 396(2-3), 293-297. https://doi.org/10.1016/0014-5793(96)01119-2

@article{c8e69602c97c4792aef7eaf515bbe212,

title = "Differentiation-dependent enhanced expression of protein phosphatase 2Cβ in germ cells of mouse seminiferous tubules",

abstract = "The presence of five distinct isoforms of protein phosphatase 2Cβ (PP2Cβ-1 ~ -5) is known. In this study, we demonstrate that the mRNA levels of PP2Cβ-3, -4 and -5 and PP2Cβ protein level increased during the course of the first wave of spermatogenesis in neonatal mouse testis. Northern blot and in situ hybridization analyses revealed that PP2Cβ-3, -4 and -5 were expressed predominantly in pachytene spermatocytes and in more highly differentiated germ cells. The substrate specificity of PP2Cβ-4 determined with artificial substrates differed from those of PP2Cβ-3 and -5, suggesting that the difference in the structure of PP2Cβ-3, -4 and -5 reflect their unique physiological functions in testicular germ cells.",

keywords = "Isoform, Protein phosphatase 2C, Spermatogenesis, Testicular germ cell",

author = "Shunsuke Kato and Takayasu Kobayashi and Kazuyuki Kusuda and Yukio Nishina and Yoshitake Nishimune and Kentaro Yomogida and Masayuki Yamamoto and Hiroyuki Sakagami and Hisatake Kondo and Motoko Ohnishi and Naoki Chida and Yuchio Yanagawa and Shinri Tamura",

note = "Funding Information: Acknowledgements.{"} We are grateful to K. Konno and F. Hayasaka for their technical assistance during the course of this study. This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Culture and Sports of Japan, Uehara Memorial Foundation and Chiba-Geigy Foundation (Japan) for the Promotion of Science.",

year = "1996",

month = nov,

day = "4",

doi = "10.1016/0014-5793(96)01119-2",

language = "English",

volume = "396",

pages = "293--297",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley-Blackwell",

number = "2-3",

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Kato, S, Kobayashi, T, Kusuda, K, Nishina, Y, Nishimune, Y, Yomogida, K, Yamamoto, M, Sakagami, H, Kondo, H, Ohnishi, M, Chida, N, Yanagawa, Y & Tamura, S 1996, 'Differentiation-dependent enhanced expression of protein phosphatase 2Cβ in germ cells of mouse seminiferous tubules', FEBS Letters, vol. 396, no. 2-3, pp. 293-297. https://doi.org/10.1016/0014-5793(96)01119-2

TY - JOUR

T1 - Differentiation-dependent enhanced expression of protein phosphatase 2Cβ in germ cells of mouse seminiferous tubules

AU - Kato, Shunsuke

AU - Kobayashi, Takayasu

AU - Kusuda, Kazuyuki

AU - Nishina, Yukio

AU - Nishimune, Yoshitake

AU - Yomogida, Kentaro

AU - Yamamoto, Masayuki

AU - Sakagami, Hiroyuki

AU - Kondo, Hisatake

AU - Ohnishi, Motoko

AU - Chida, Naoki

AU - Yanagawa, Yuchio

AU - Tamura, Shinri

N1 - Funding Information: Acknowledgements." We are grateful to K. Konno and F. Hayasaka for their technical assistance during the course of this study. This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Culture and Sports of Japan, Uehara Memorial Foundation and Chiba-Geigy Foundation (Japan) for the Promotion of Science.

PY - 1996/11/4

Y1 - 1996/11/4

N2 - The presence of five distinct isoforms of protein phosphatase 2Cβ (PP2Cβ-1 ~ -5) is known. In this study, we demonstrate that the mRNA levels of PP2Cβ-3, -4 and -5 and PP2Cβ protein level increased during the course of the first wave of spermatogenesis in neonatal mouse testis. Northern blot and in situ hybridization analyses revealed that PP2Cβ-3, -4 and -5 were expressed predominantly in pachytene spermatocytes and in more highly differentiated germ cells. The substrate specificity of PP2Cβ-4 determined with artificial substrates differed from those of PP2Cβ-3 and -5, suggesting that the difference in the structure of PP2Cβ-3, -4 and -5 reflect their unique physiological functions in testicular germ cells.

AB - The presence of five distinct isoforms of protein phosphatase 2Cβ (PP2Cβ-1 ~ -5) is known. In this study, we demonstrate that the mRNA levels of PP2Cβ-3, -4 and -5 and PP2Cβ protein level increased during the course of the first wave of spermatogenesis in neonatal mouse testis. Northern blot and in situ hybridization analyses revealed that PP2Cβ-3, -4 and -5 were expressed predominantly in pachytene spermatocytes and in more highly differentiated germ cells. The substrate specificity of PP2Cβ-4 determined with artificial substrates differed from those of PP2Cβ-3 and -5, suggesting that the difference in the structure of PP2Cβ-3, -4 and -5 reflect their unique physiological functions in testicular germ cells.

KW - Isoform

KW - Protein phosphatase 2C

KW - Spermatogenesis

KW - Testicular germ cell

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U2 - 10.1016/0014-5793(96)01119-2

DO - 10.1016/0014-5793(96)01119-2

M3 - Article

C2 - 8915006

AN - SCOPUS:0030569396

SN - 0014-5793

VL - 396

SP - 293

EP - 297

JO - FEBS Letters

JF - FEBS Letters

IS - 2-3

ER -

Differentiation-dependent enhanced expression of protein phosphatase 2Cβ in germ cells of mouse seminiferous tubules

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