TY - JOUR
T1 - Differentiation of Veillonella atypica, Veillonella dispar and Veillonella parvula using restricted fragment-length polymorphism analysis of 16S rDNA amplified by polymerase chain reaction
AU - Sato, T.
AU - Matsuyama, J.
AU - Sato, M.
AU - Hoshino, E.
PY - 1997/12
Y1 - 1997/12
N2 - Veillonella atypica, Veillonella dispar and Veillonella parvula cannot be reliably distinguished by conventional phenotypic tests, including the API ZYM test. In this study, restricted fragment-length polymorphism (RFLP) analysis of 16S rDNA amplified by polymerase chain reaction (PCR) was used to generate restriction profiles of the type strains of V. atypica, V. dispar and V. parvula and 20 Veillonella strains isolated from oral sites. 16S rRNA gene sequences from isolated genomic DNA samples were amplified by PCR. PCR products were purified and characterized by single digestion with 13 restriction endonucleases. Among them, MnlI was found to discriminate the respective reference strains, and the clinical isolates were assigned to one of the three species on the basis of their restriction profiles by digestion with MnlI. Thus, RFLP analysis of PCR-amplified 16S rDNA, using MnlI, is a rapid and reliable method for the differentiation of V. atypica, V. dispar and V. parvula.
AB - Veillonella atypica, Veillonella dispar and Veillonella parvula cannot be reliably distinguished by conventional phenotypic tests, including the API ZYM test. In this study, restricted fragment-length polymorphism (RFLP) analysis of 16S rDNA amplified by polymerase chain reaction (PCR) was used to generate restriction profiles of the type strains of V. atypica, V. dispar and V. parvula and 20 Veillonella strains isolated from oral sites. 16S rRNA gene sequences from isolated genomic DNA samples were amplified by PCR. PCR products were purified and characterized by single digestion with 13 restriction endonucleases. Among them, MnlI was found to discriminate the respective reference strains, and the clinical isolates were assigned to one of the three species on the basis of their restriction profiles by digestion with MnlI. Thus, RFLP analysis of PCR-amplified 16S rDNA, using MnlI, is a rapid and reliable method for the differentiation of V. atypica, V. dispar and V. parvula.
KW - 16S rDNA
KW - PCR-RFLP
KW - Veillonella atypica
KW - Veillonella dispar
KW - Veillonella parvula
UR - http://www.scopus.com/inward/record.url?scp=0031543453&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031543453&partnerID=8YFLogxK
U2 - 10.1111/j.1399-302X.1997.tb00737.x
DO - 10.1111/j.1399-302X.1997.tb00737.x
M3 - Article
C2 - 9573809
AN - SCOPUS:0031543453
SN - 2041-1006
VL - 12
SP - 350
EP - 353
JO - Molecular Oral Microbiology
JF - Molecular Oral Microbiology
IS - 6
ER -