TY - JOUR
T1 - Direct evidence of in vivo nitric oxide production and inducible nitric oxide synthase mRNA expression in the brain of living rat during experimental meningitis
AU - Suzuki, Yasuhiro
AU - Fujii, Satoshi
AU - Tominaga, Teiji
AU - Yoshimoto, Takashi
AU - Fujii, Shigemoto
AU - Akaike, Takaaki
AU - Maeda, Hiroshi
AU - Yoshimura, Tetsuhiko
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1999
Y1 - 1999
N2 - To detect endogenous nitric oxide (NO) produced in a rat bacterial meningitis model, the authors applied an electron paramagnetic resonance (EPR) NO-trapping technique. Iron complex with N,N-diethyldithiocarbamate were used as a trapping agent. Experimental meningitis was induced by a mixture of lipopolysaccharide and interferon-γ. Sequential changes of NO formation under meningitis were observed in rat brain tissue by using X-band (9 GHz) EPR spectroscopy, and endogenous NO was detected in the head of a living rat with a 700-MHz EPR system. Inducible NO synthase mRNA expression in the brain tissues also was proven by using a reverse transcriptase- polymerase chain reaction technique.
AB - To detect endogenous nitric oxide (NO) produced in a rat bacterial meningitis model, the authors applied an electron paramagnetic resonance (EPR) NO-trapping technique. Iron complex with N,N-diethyldithiocarbamate were used as a trapping agent. Experimental meningitis was induced by a mixture of lipopolysaccharide and interferon-γ. Sequential changes of NO formation under meningitis were observed in rat brain tissue by using X-band (9 GHz) EPR spectroscopy, and endogenous NO was detected in the head of a living rat with a 700-MHz EPR system. Inducible NO synthase mRNA expression in the brain tissues also was proven by using a reverse transcriptase- polymerase chain reaction technique.
KW - In vivo electron paramagnetic resonance detection
KW - Inducible nitric oxide synthase mRNA
KW - Meningitis
KW - Nitric oxide
KW - Spin-Trapping
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U2 - 10.1097/00004647-199911000-00001
DO - 10.1097/00004647-199911000-00001
M3 - Article
C2 - 10566963
AN - SCOPUS:0033496985
SN - 0271-678X
VL - 19
SP - 1175
EP - 1178
JO - Journal of Cerebral Blood Flow and Metabolism
JF - Journal of Cerebral Blood Flow and Metabolism
IS - 11
ER -