TY - JOUR
T1 - Dispersion and degradation of environmental DNA from caged fish in a marine environment
AU - Murakami, Hiroaki
AU - Yoon, Seokjin
AU - Kasai, Akihide
AU - Minamoto, Toshifumi
AU - Yamamoto, Satoshi
AU - Sakata, Masayuki K.
AU - Horiuchi, Tomoya
AU - Sawada, Hideki
AU - Kondoh, Michio
AU - Yamashita, Yoh
AU - Masuda, Reiji
N1 - Funding Information:
Acknowledgements We thank Yoshihito Ogura for navigating the boat and Masahiro Mukai and Aina Tanimoto (MFRS) for assisting with filtration procedures. This study was supported by CREST of JST (grant number: JPMJCR13A2) and the Sasakawa Scientific Research Grant from The Japan Science Society.
Publisher Copyright:
© 2019, Japanese Society of Fisheries Science.
PY - 2019/3/15
Y1 - 2019/3/15
N2 - Environmental DNA (eDNA) consists of DNA fragments shed from organisms into the environment, and can be used to identify species presence and abundance. This study aimed to reveal the dispersion and degradation processes of eDNA in the sea. Caged fish were set off the end of a pier in Maizuru Bay, the Sea of Japan, and their eDNA was traced at sampling stations located at the cage and 10, 30, 100, 300, 600 and 1000 m distances from the cage along two transect lines. Sea surface water was collected at each station at 0, 2, 4, 8, 24 and 48 h after setting the cage, and again after removing the cage. Quantitative PCR analyses using a species-specific primer and probe set revealed that the target DNA was detectable while the cage was present and for up to 1 h after removing the cage, but not at 2 h or later. Among the 57 amplified samples, 45 (79%) were collected within 30 m from the cage. These results suggest that eDNA can provide a snapshot of organisms present in a coastal marine environment.
AB - Environmental DNA (eDNA) consists of DNA fragments shed from organisms into the environment, and can be used to identify species presence and abundance. This study aimed to reveal the dispersion and degradation processes of eDNA in the sea. Caged fish were set off the end of a pier in Maizuru Bay, the Sea of Japan, and their eDNA was traced at sampling stations located at the cage and 10, 30, 100, 300, 600 and 1000 m distances from the cage along two transect lines. Sea surface water was collected at each station at 0, 2, 4, 8, 24 and 48 h after setting the cage, and again after removing the cage. Quantitative PCR analyses using a species-specific primer and probe set revealed that the target DNA was detectable while the cage was present and for up to 1 h after removing the cage, but not at 2 h or later. Among the 57 amplified samples, 45 (79%) were collected within 30 m from the cage. These results suggest that eDNA can provide a snapshot of organisms present in a coastal marine environment.
KW - Detectability
KW - Quantitative PCR
KW - Species-specific primers and probe
KW - Striped jack Pseudocaranx dentex
KW - Transport
KW - eDNA
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U2 - 10.1007/s12562-018-1282-6
DO - 10.1007/s12562-018-1282-6
M3 - Article
AN - SCOPUS:85059523673
SN - 0919-9268
VL - 85
SP - 327
EP - 337
JO - Fisheries Science
JF - Fisheries Science
IS - 2
ER -
