Dissociation of HSP72 and HSC73 heat shock mRNA inductions after spinal cord ischemia in rabbit

Masahiro Sakurai; Masashi Aoki; Koji Abe; Mitsuaki Sadahiro; Koichi Tabayashi

doi:10.1016/0304-3940(96)13079-2

Dissociation of HSP72 and HSC73 heat shock mRNA inductions after spinal cord ischemia in rabbit

Masahiro Sakurai, Masashi Aoki, Koji Abe, Mitsuaki Sadahiro, Koichi Tabayashi

MED - Medical Sciences

Tohoku University

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The inductions of HSP72 and HSC73 mRNAs were investigated with Northern blot and immunohistochemical analyses for up to 7 days of the reperfusion after 5 or 15 min ischemia in rabbit spinal cord. Following 15 min ischemia, HSP72 mRNA was induced at 8 h of reperfusion, while HSC73 mRNA continuously remained at the control level. Immunoreactivity of HSP72 protein was slightly induced at 8 h of reperfusion selectively in motor neurons, and the majority of motor neuron showed selective cell death at 7 days of reperfusion. This study demonstrated selective induction of HSP72 mRNA and the protein in motor neuron cells that eventually showed selective delayed neuronal death after transient ischemia in the spinal cord. This phenomenon was not accompanied by HSC73 induction.

Original language	English
Pages (from-to)	113-116
Number of pages	4
Journal	Neuroscience Letters
Volume	217
Issue number	2-3
DOIs	https://doi.org/10.1016/0304-3940(96)13079-2
Publication status	Published - 1996 Oct 18

Keywords

Heat shock protein
Rabbit
Spinal cord ischemia

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10.1016/0304-3940(96)13079-2

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title = "Dissociation of HSP72 and HSC73 heat shock mRNA inductions after spinal cord ischemia in rabbit",

abstract = "The inductions of HSP72 and HSC73 mRNAs were investigated with Northern blot and immunohistochemical analyses for up to 7 days of the reperfusion after 5 or 15 min ischemia in rabbit spinal cord. Following 15 min ischemia, HSP72 mRNA was induced at 8 h of reperfusion, while HSC73 mRNA continuously remained at the control level. Immunoreactivity of HSP72 protein was slightly induced at 8 h of reperfusion selectively in motor neurons, and the majority of motor neuron showed selective cell death at 7 days of reperfusion. This study demonstrated selective induction of HSP72 mRNA and the protein in motor neuron cells that eventually showed selective delayed neuronal death after transient ischemia in the spinal cord. This phenomenon was not accompanied by HSC73 induction.",

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AU - Sakurai, Masahiro

AU - Aoki, Masashi

AU - Abe, Koji

AU - Sadahiro, Mitsuaki

AU - Tabayashi, Koichi

PY - 1996/10/18

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AB - The inductions of HSP72 and HSC73 mRNAs were investigated with Northern blot and immunohistochemical analyses for up to 7 days of the reperfusion after 5 or 15 min ischemia in rabbit spinal cord. Following 15 min ischemia, HSP72 mRNA was induced at 8 h of reperfusion, while HSC73 mRNA continuously remained at the control level. Immunoreactivity of HSP72 protein was slightly induced at 8 h of reperfusion selectively in motor neurons, and the majority of motor neuron showed selective cell death at 7 days of reperfusion. This study demonstrated selective induction of HSP72 mRNA and the protein in motor neuron cells that eventually showed selective delayed neuronal death after transient ischemia in the spinal cord. This phenomenon was not accompanied by HSC73 induction.

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KW - Rabbit

KW - Spinal cord ischemia

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Dissociation of HSP72 and HSC73 heat shock mRNA inductions after spinal cord ischemia in rabbit

Abstract

Keywords

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