TY - JOUR
T1 - Distinct spatiotemporal expression of EFA6D, a guanine nucleotide exchange factor for ARF6, among the EFA6 family in mouse brain
AU - Sakagami, Hiroyuki
AU - Suzuki, Hiroharu
AU - Kamata, Akifumi
AU - Owada, Yuji
AU - Fukunaga, Kohji
AU - Mayanagi, Hideaki
AU - Kondo, Hisatake
N1 - Funding Information:
The authors would like to thank Dr. J. Miyazaki (Osaka University Medical School) for kindly providing pCAGGS vector, Drs. Hye-Won Shin and Kazuhisa Nakayama (Kyoto University Graduate School of Pharmaceutical Sciences) for the expression vectors for ARFs and technical advice on ARF pull-down assay, and Dr. Hiroshi Takeshima (Tohoku University Graduate School of Medicine) for mouse cDNA libraries. This work is supported by a Grant-in-Aid for Scientific Research (C) (#17500219) to H.S. from the Ministry of Education, Science, Sports, Culture and Technology of Japan.
PY - 2006/6/6
Y1 - 2006/6/6
N2 - The EFA6 family is a member of guanine nucleotide exchange factors (GEFs) that can activate ARF6 specifically in vitro. In this study, we determined the complete primary sequence of mouse EFA6D encoding a protein of 1004 amino acids with a calculated molecular weight of 111,207 Da. In ARF pull-down assay, EFA6D showed a preferential GEF activity toward ARF6. RT-PCR analysis revealed the widespread tissue distribution of EFA6D and the high expression of EFA6A, C and D in the brain. In situ hybridization analysis demonstrated a distinct spatiotemporal expression pattern of EFA6D from those of EFA6A and C in mouse brain. Furthermore, immunoblot analysis revealed that EFA6D was highly concentrated in the postsynaptic density fraction. These findings suggest differential spatiotemporal regulation of ARF6 by three members of the EFA6 family in the brain.
AB - The EFA6 family is a member of guanine nucleotide exchange factors (GEFs) that can activate ARF6 specifically in vitro. In this study, we determined the complete primary sequence of mouse EFA6D encoding a protein of 1004 amino acids with a calculated molecular weight of 111,207 Da. In ARF pull-down assay, EFA6D showed a preferential GEF activity toward ARF6. RT-PCR analysis revealed the widespread tissue distribution of EFA6D and the high expression of EFA6A, C and D in the brain. In situ hybridization analysis demonstrated a distinct spatiotemporal expression pattern of EFA6D from those of EFA6A and C in mouse brain. Furthermore, immunoblot analysis revealed that EFA6D was highly concentrated in the postsynaptic density fraction. These findings suggest differential spatiotemporal regulation of ARF6 by three members of the EFA6 family in the brain.
KW - ADP-ribosylation factor 6
KW - Brain
KW - Development
KW - EFA6
KW - Guanine nucleotide exchange protein
KW - Mouse
KW - Postsynaptic density
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U2 - 10.1016/j.brainres.2006.02.058
DO - 10.1016/j.brainres.2006.02.058
M3 - Article
C2 - 16707115
AN - SCOPUS:33745363517
SN - 0006-8993
VL - 1093
SP - 1
EP - 11
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1
ER -