Distribution of Amniotic Epithelial Cells After Intraportal Infusion in a Rat Model

Miyako Tanaka, Kazuaki Tokodai, Masato Sato, Shuhei Yamada, Hitomi Okita, Takako Ito, Masatoshi Saito, Tetsuro Hoshiai, Shigehito Miyagi, Toshio Miki, Michiaki Unno, Takashi Kamei, Masafumi Goto

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Background: Human amniotic epithelial cells (hAECs) are increasingly gaining attention as novel sources for cell transplantation. In clinical practice, intraportal infusion is considered one of the leading approaches for transplantation; however, this has not yet been validated for in vivo transplantation of hAECs. Thus, this study aims to investigate the distribution of hAECs post intraportal infusion and compare this distribution with other cell administration routes. Methods: Wistar/ST rats were divided into 4 groups (n = 3 for each) based on cell administration route: group 1, intraportal; group 2, the spleen; group 3, tail veins; and group 4, penile veins. Subsequently, hAECs (1 × 107) stained with XenoLight DiR were infused into each recipient. Cell distribution was evaluated using an in vivo imaging system. Results: DiR signals were detected in the rat livers of groups 1 and 2 with those in group 2 being much weaker than those in group 1. Necrosis of small intestine was observed in 2 cases in group 2. DiR signals were detected in the lungs in groups 3 and 4 because of systemic circulation; however, all the animals died within 20 minutes of infusions. Conclusions: Intraportal infusion is potentially applicable for safe and efficient transplantation of hAECs into the liver, whereas hAECs administration via the spleen carries a risk of thrombosis in a narrow portal vein system. Our results also indicate that hAECs administration via the systemic circulation could cause pulmonary embolism in clinical settings.

Original languageEnglish
Pages (from-to)513-515
Number of pages3
JournalTransplantation Proceedings
Issue number2
Publication statusPublished - 2022 Mar

ASJC Scopus subject areas

  • Surgery
  • Transplantation


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