Distribution of Cryptosporidium species isolated from diarrhoeic calves in Japan

Mohammad Hazzaz Bin Kabir; Megumi Itoh; Ayman Ahmed Shehata; Hironori Bando; Yasuhiro Fukuda; Fumi Murakoshi; Atsushi Fujikura; Hiroaki Okawa; Takuto Endo; Akira Goto; Masayuki Kachi; Toshie Nakayama; Yuto Kano; Shoko Oishi; Konosuke Otomaru; Mohamed Ibrahim Essa; Kei Kazama; Xuenan Xuan; Kentaro Kato

doi:10.1016/j.parint.2020.102153

Distribution of Cryptosporidium species isolated from diarrhoeic calves in Japan

Mohammad Hazzaz Bin Kabir, Megumi Itoh, Ayman Ahmed Shehata, Hironori Bando, Yasuhiro Fukuda, Fumi Murakoshi, Atsushi Fujikura, Hiroaki Okawa, Takuto Endo, Akira Goto, Masayuki Kachi, Toshie Nakayama, Yuto Kano, Shoko Oishi, Konosuke Otomaru, Mohamed Ibrahim Essa, Kei Kazama, Xuenan Xuan, Kentaro Kato

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Cryptosporidium spp. are enteric protozoan parasites that infect a wide range of hosts including humans, and domestic and wild animals. The aim of this study was to molecularly characterize the Cryptosporidium spp. found in calf faeces in Japan. A total of 80 pre-weaned beef and dairy calves' diarrhoeic faecal specimens were collected from nine different prefectures in Japan. A nested polymerase chain reaction targeting the small subunit 18S rRNA and GP60 genes were used to detect the Cryptosporidium genotypes and subtypes. 83.8% (67 out of 80) of the specimens were positive for Cryptosporidium spp.; Cryptosporidium was found in both beef and dairy calves. Cryptosporidium parvum was the predominant species, detected in 77.5% (31/40) of beef calves and 80% (32/40) of dairy calves. Cryptosporidium bovis was also detected, 5.0% (2/40) of dairy calves, and C. ryanae was also found 2.5% (1/40) of dairy calves. One mixed-species infection, 2.5% (1/40) was detected in a beef calf having C. parvum, and C. ryanae. We detected the most common subtype of C. parvum (i.e., IIaA15G2R1), as well as other subtypes (i.e., IIaA14G3R1, IIaA14G2R1, and IIaA13G1R1) that have not previously been detected in calves in Japan. Our results demonstrate the widespread diversity of Cryptosporidium infection in calves in Japan.

Original language	English
Article number	102153
Journal	Parasitology International
Volume	78
DOIs	https://doi.org/10.1016/j.parint.2020.102153
Publication status	Published - 2020 Oct

Keywords

C. parvum
Cryptosporidium
Japan
PCR-based sequencing
Phylogenetic analyses
Pre-weaned calves

ASJC Scopus subject areas

Parasitology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.parint.2020.102153

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Kabir, M. H. B., Itoh, M., Shehata, A. A., Bando, H., Fukuda, Y., Murakoshi, F., Fujikura, A., Okawa, H., Endo, T., Goto, A., Kachi, M., Nakayama, T., Kano, Y., Oishi, S., Otomaru, K., Essa, M. I., Kazama, K., Xuan, X., & Kato, K. (2020). Distribution of Cryptosporidium species isolated from diarrhoeic calves in Japan. Parasitology International, 78, [102153]. https://doi.org/10.1016/j.parint.2020.102153

Kabir, MHB, Itoh, M, Shehata, AA, Bando, H, Fukuda, Y, Murakoshi, F, Fujikura, A, Okawa, H, Endo, T, Goto, A, Kachi, M, Nakayama, T, Kano, Y, Oishi, S, Otomaru, K, Essa, MI, Kazama, K, Xuan, X & Kato, K 2020, 'Distribution of Cryptosporidium species isolated from diarrhoeic calves in Japan', Parasitology International, vol. 78, 102153. https://doi.org/10.1016/j.parint.2020.102153

@article{2785c371e3c341d5b8a0284e5ab2d239,

title = "Distribution of Cryptosporidium species isolated from diarrhoeic calves in Japan",

abstract = "Cryptosporidium spp. are enteric protozoan parasites that infect a wide range of hosts including humans, and domestic and wild animals. The aim of this study was to molecularly characterize the Cryptosporidium spp. found in calf faeces in Japan. A total of 80 pre-weaned beef and dairy calves' diarrhoeic faecal specimens were collected from nine different prefectures in Japan. A nested polymerase chain reaction targeting the small subunit 18S rRNA and GP60 genes were used to detect the Cryptosporidium genotypes and subtypes. 83.8% (67 out of 80) of the specimens were positive for Cryptosporidium spp.; Cryptosporidium was found in both beef and dairy calves. Cryptosporidium parvum was the predominant species, detected in 77.5% (31/40) of beef calves and 80% (32/40) of dairy calves. Cryptosporidium bovis was also detected, 5.0% (2/40) of dairy calves, and C. ryanae was also found 2.5% (1/40) of dairy calves. One mixed-species infection, 2.5% (1/40) was detected in a beef calf having C. parvum, and C. ryanae. We detected the most common subtype of C. parvum (i.e., IIaA15G2R1), as well as other subtypes (i.e., IIaA14G3R1, IIaA14G2R1, and IIaA13G1R1) that have not previously been detected in calves in Japan. Our results demonstrate the widespread diversity of Cryptosporidium infection in calves in Japan.",

keywords = "C. parvum, Cryptosporidium, Japan, PCR-based sequencing, Phylogenetic analyses, Pre-weaned calves",

author = "Kabir, {Mohammad Hazzaz Bin} and Megumi Itoh and Shehata, {Ayman Ahmed} and Hironori Bando and Yasuhiro Fukuda and Fumi Murakoshi and Atsushi Fujikura and Hiroaki Okawa and Takuto Endo and Akira Goto and Masayuki Kachi and Toshie Nakayama and Yuto Kano and Shoko Oishi and Konosuke Otomaru and Essa, {Mohamed Ibrahim} and Kei Kazama and Xuenan Xuan and Kentaro Kato",

note = "Funding Information: This study was funded by grants-in-aid for Scientific Research ( B:17H03913 ) and ( C:16KT0141 ) and Scientific Research on Innovative Areas ( 3805 ) from the Ministry of Education, Culture, Science, Sports, and Technology (MEXT) of Japan , and by a Livestock Promotional Subsidy from the Japan Racing Association . Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",

year = "2020",

month = oct,

doi = "10.1016/j.parint.2020.102153",

language = "English",

volume = "78",

journal = "Parasitology International",

issn = "1383-5769",

publisher = "Elsevier Ireland Ltd",

}

TY - JOUR

T1 - Distribution of Cryptosporidium species isolated from diarrhoeic calves in Japan

AU - Kabir, Mohammad Hazzaz Bin

AU - Itoh, Megumi

AU - Shehata, Ayman Ahmed

AU - Bando, Hironori

AU - Fukuda, Yasuhiro

AU - Murakoshi, Fumi

AU - Fujikura, Atsushi

AU - Okawa, Hiroaki

AU - Endo, Takuto

AU - Goto, Akira

AU - Kachi, Masayuki

AU - Nakayama, Toshie

AU - Kano, Yuto

AU - Oishi, Shoko

AU - Otomaru, Konosuke

AU - Essa, Mohamed Ibrahim

AU - Kazama, Kei

AU - Xuan, Xuenan

AU - Kato, Kentaro

N1 - Funding Information: This study was funded by grants-in-aid for Scientific Research ( B:17H03913 ) and ( C:16KT0141 ) and Scientific Research on Innovative Areas ( 3805 ) from the Ministry of Education, Culture, Science, Sports, and Technology (MEXT) of Japan , and by a Livestock Promotional Subsidy from the Japan Racing Association . Publisher Copyright: © 2020 Elsevier B.V.

PY - 2020/10

Y1 - 2020/10

N2 - Cryptosporidium spp. are enteric protozoan parasites that infect a wide range of hosts including humans, and domestic and wild animals. The aim of this study was to molecularly characterize the Cryptosporidium spp. found in calf faeces in Japan. A total of 80 pre-weaned beef and dairy calves' diarrhoeic faecal specimens were collected from nine different prefectures in Japan. A nested polymerase chain reaction targeting the small subunit 18S rRNA and GP60 genes were used to detect the Cryptosporidium genotypes and subtypes. 83.8% (67 out of 80) of the specimens were positive for Cryptosporidium spp.; Cryptosporidium was found in both beef and dairy calves. Cryptosporidium parvum was the predominant species, detected in 77.5% (31/40) of beef calves and 80% (32/40) of dairy calves. Cryptosporidium bovis was also detected, 5.0% (2/40) of dairy calves, and C. ryanae was also found 2.5% (1/40) of dairy calves. One mixed-species infection, 2.5% (1/40) was detected in a beef calf having C. parvum, and C. ryanae. We detected the most common subtype of C. parvum (i.e., IIaA15G2R1), as well as other subtypes (i.e., IIaA14G3R1, IIaA14G2R1, and IIaA13G1R1) that have not previously been detected in calves in Japan. Our results demonstrate the widespread diversity of Cryptosporidium infection in calves in Japan.

AB - Cryptosporidium spp. are enteric protozoan parasites that infect a wide range of hosts including humans, and domestic and wild animals. The aim of this study was to molecularly characterize the Cryptosporidium spp. found in calf faeces in Japan. A total of 80 pre-weaned beef and dairy calves' diarrhoeic faecal specimens were collected from nine different prefectures in Japan. A nested polymerase chain reaction targeting the small subunit 18S rRNA and GP60 genes were used to detect the Cryptosporidium genotypes and subtypes. 83.8% (67 out of 80) of the specimens were positive for Cryptosporidium spp.; Cryptosporidium was found in both beef and dairy calves. Cryptosporidium parvum was the predominant species, detected in 77.5% (31/40) of beef calves and 80% (32/40) of dairy calves. Cryptosporidium bovis was also detected, 5.0% (2/40) of dairy calves, and C. ryanae was also found 2.5% (1/40) of dairy calves. One mixed-species infection, 2.5% (1/40) was detected in a beef calf having C. parvum, and C. ryanae. We detected the most common subtype of C. parvum (i.e., IIaA15G2R1), as well as other subtypes (i.e., IIaA14G3R1, IIaA14G2R1, and IIaA13G1R1) that have not previously been detected in calves in Japan. Our results demonstrate the widespread diversity of Cryptosporidium infection in calves in Japan.

KW - C. parvum

KW - Cryptosporidium

KW - Japan

KW - PCR-based sequencing

KW - Phylogenetic analyses

KW - Pre-weaned calves

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DO - 10.1016/j.parint.2020.102153

M3 - Article

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AN - SCOPUS:85086757779

SN - 1383-5769

VL - 78

JO - Parasitology International

JF - Parasitology International

M1 - 102153

ER -

Distribution of Cryptosporidium species isolated from diarrhoeic calves in Japan

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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