TY - JOUR
T1 - Distribution of tetrodotoxin, saxitoxin, and their analogs among tissues of the puffer fish Fugu pardalis
AU - Jang, Junho
AU - Yotsu-Yamashita, Mari
N1 - Funding Information:
We are grateful to Dr. Oshima, Professor of Tohoku University for kind supply of PSP toxin standards. This work was supported by Nagase Science and Technology Foundation, and by Grants-in-Aid for Scientific Research on Priority Areas from MEXT (18032014), and from JSPS (17580091).
PY - 2006/12/15
Y1 - 2006/12/15
N2 - The anatomical distribution of tetrodotoxin (TTX), saxitoxin (STX) and their analogs (TTXs, STXs) in three female and three male specimens of the marine puffer fish Fugu pardalis from Miyagi Prefecture, 2005, Japan, were studied. 5-DeoxyTTX, 11-deoxyTTX, and 5,6,11-trideoxyTTX were quantified by liquid chromatography/mass spectrometry (LC/MS) for the first time, and other TTXs and STXs were determined by liquid chromatography-fluorescent detection (LC-FLD). As a result, 5,6,11-trideoxyTTX was found to be the major TTX analog in all tissues tested, whereas 5-deoxyTTX and 11-deoxyTTX were minor components. Especially, in female (n = 3), the ratios of 5,6,11-trideoxyTTX to total of all TTX analogs (mole/mole) in ovaries (mean±SD, 0.42±0.055) were significantly larger than those in livers (0.17±0.025) (P < 0.05). In contrary, the ratios of 4,9-anhydroTTX to total of all TTX analogs in livers (0.27±0.047) were significantly larger than those in ovaries (0.073±0.040) (P < 0.01). The ratios of TTX to total of all TTX analogs were not significantly different between ovaries (0.47±0.078) and livers (0.55±0.067). In male (n = 3), all these ratios were not significantly different between livers and testis. 4-S-CysteinylTTX was detected in liver, spleen, gall, and intestine in 1-6 mole% of total of all TTX analogs, supporting our previous hypothesis that 4-S-cysteinylTTX is a metabolite of TTX.
AB - The anatomical distribution of tetrodotoxin (TTX), saxitoxin (STX) and their analogs (TTXs, STXs) in three female and three male specimens of the marine puffer fish Fugu pardalis from Miyagi Prefecture, 2005, Japan, were studied. 5-DeoxyTTX, 11-deoxyTTX, and 5,6,11-trideoxyTTX were quantified by liquid chromatography/mass spectrometry (LC/MS) for the first time, and other TTXs and STXs were determined by liquid chromatography-fluorescent detection (LC-FLD). As a result, 5,6,11-trideoxyTTX was found to be the major TTX analog in all tissues tested, whereas 5-deoxyTTX and 11-deoxyTTX were minor components. Especially, in female (n = 3), the ratios of 5,6,11-trideoxyTTX to total of all TTX analogs (mole/mole) in ovaries (mean±SD, 0.42±0.055) were significantly larger than those in livers (0.17±0.025) (P < 0.05). In contrary, the ratios of 4,9-anhydroTTX to total of all TTX analogs in livers (0.27±0.047) were significantly larger than those in ovaries (0.073±0.040) (P < 0.01). The ratios of TTX to total of all TTX analogs were not significantly different between ovaries (0.47±0.078) and livers (0.55±0.067). In male (n = 3), all these ratios were not significantly different between livers and testis. 4-S-CysteinylTTX was detected in liver, spleen, gall, and intestine in 1-6 mole% of total of all TTX analogs, supporting our previous hypothesis that 4-S-cysteinylTTX is a metabolite of TTX.
KW - LC/MS
KW - Puffer fish
KW - Saxitoxin
KW - Tetrodotoxin
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U2 - 10.1016/j.toxicon.2006.07.034
DO - 10.1016/j.toxicon.2006.07.034
M3 - Article
C2 - 16997342
AN - SCOPUS:33750931583
SN - 0041-0101
VL - 48
SP - 980
EP - 987
JO - Toxicon
JF - Toxicon
IS - 8
ER -
