Diversity of site-specific microbes of occlusal and proximal lesions in severe- early childhood caries (S-ECC)

Kausar Sadia Fakhruddin, Lakshman Perera Samaranayake, Rifat Akram Hamoudi, Hien Chi Ngo, Hiroshi Egusa

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5 Citations (Scopus)

Abstract

Background: Severe-early childhood caries (S-ECC) a global problem of significant concern, commonly manifest on the occlusal, and proximal surfaces of affected teeth. Despite the major ecological differences between these two niches the compositional differences, if any, in the microbiota of such lesions is unknown. Methods: Deep-dentine caries samples from asymptomatic primary molars of children with S-ECC (n 19) belonging to caries-code 5/6, (ICDAS classification) were evaluated. Employing two primer pools, we amplified and compared the bacterial 16S rRNA gene sequences of the seven hypervariable regions (V2—V4 and V6—V9) using NGS-based assay. Results: Bray-Curtisevaluation indicated that occlusal lesions (OL) had a more homogeneous community than the proximal lesions (PL) with significant compositional differences at the species level (p = 0.01; R- 0.513). Together, the occlusal and proximal niches harbored 263 species, of which 202 (76.8%) species were common to both, while 49 (18.6%) and 12 (4.6%) disparate species were exclusively isolated from the proximal and occlusal niches, respectively. The most commonl genera at both niches included Streptococcus, Prevotella, and Lactobacillus. S. mutans was predominant in PL (p ≤ 0.05), and Atopobium parvulum (p = 0.01) was predominant in OL. Conclusions: Distinct differences exist between the caries microbiota of occlusal and proximal caries in S-ECC.

Original languageEnglish
Article number2037832
JournalJournal of Oral Microbiology
Volume14
Issue number1
DOIs
Publication statusPublished - 2022

Keywords

  • dentine caries
  • microbial diversity
  • occlusal-caries lesion
  • proximal-caries lesion
  • Severe-early childhood caries (S-ECC)

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