DNA fragmentation in focal cortical freeze injury of rats

Teiji Tominaga, Shigeo Kure, Takashi Yoshimoto

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)


This study examined the appearance of double-strand DNA breaks in rat brain after a focal cortical freeze injury in vivo. DNA fragments of oligonucleosome size appeared 3 h after the injury, and increased in a time-dependent manner. At 24 h, the amount of DNA fragmentation reached a maximum and then declined. When nuclei from freeze-injured brain tissue were incubated with Ca2+ in vitro, increased endonuclease activity, which can cause DNA fragmentation, was found. These findings indicate that the activation of a Ca2+-dependent endonuclease may be involved in the evolution of freeze-traumatized brain tissue.

Original languageEnglish
Pages (from-to)265-268
Number of pages4
JournalNeuroscience Letters
Issue number2
Publication statusPublished - 1992 May 25


  • Brain nucleus
  • Calcium
  • DNA fragmentation
  • Endonuclease
  • Freeze injury

ASJC Scopus subject areas

  • Neuroscience(all)


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