DNA hybridization detection by porous silicon-based DNA microarray in conjugation with infrared microspectroscopy

Ryo Taro Yamaguchi; Ko Ichiro Miyamoto; Ken Ichi Ishibashi; Ayumi Hirano; Suhana Mohd Said; Yasuo Kimura; Michio Niwano

doi:10.1063/1.2751415

DNA hybridization detection by porous silicon-based DNA microarray in conjugation with infrared microspectroscopy

Ryo Taro Yamaguchi, Ko Ichiro Miyamoto, Ken Ichi Ishibashi, Ayumi Hirano, Suhana Mohd Said, Yasuo Kimura, Michio Niwano

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21 Citations (Scopus)

Abstract

A method is described for the label-free detection of DNA hybridization on porous silicon (por-Si), based upon the pairing of oligonucleotide chemistry and standard silicon nanotechnology. Por-Si with a pore diameter of approximately 30 nm was used to immobilize probe DNA. Infrared microspectroscopy was employed to monitor the hybridization of probe-DNA immobilized on pore surfaces with its complementary DNA (target-DNA). The immobilization of probe DNA on por-Si facilitates hybridization detection for a small sensing area (approximately 50×50 μ m2) with a high detection efficiency. In this study, we fabricated a porous silicon-based DNA microarray (por-Si-microarray) using photolithographic and Si anodizing techniques. We demonstrate that DNA hybridization can be detected on a por-Si-microarray through the analysis of infrared absorption spectral profiles in the region where the vibration modes of the bases appear. This present approach demonstrates that por-Si-microarray in conjugation with infrared microspectroscopy has potential application in DNA sensing chips.

Original language	English
Article number	014303
Journal	Journal of Applied Physics
Volume	102
Issue number	1
DOIs	https://doi.org/10.1063/1.2751415
Publication status	Published - 2007

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@article{01fb5a24155c4d2e9dfbd25dece04112,

title = "DNA hybridization detection by porous silicon-based DNA microarray in conjugation with infrared microspectroscopy",

abstract = "A method is described for the label-free detection of DNA hybridization on porous silicon (por-Si), based upon the pairing of oligonucleotide chemistry and standard silicon nanotechnology. Por-Si with a pore diameter of approximately 30 nm was used to immobilize probe DNA. Infrared microspectroscopy was employed to monitor the hybridization of probe-DNA immobilized on pore surfaces with its complementary DNA (target-DNA). The immobilization of probe DNA on por-Si facilitates hybridization detection for a small sensing area (approximately 50×50 μ m2) with a high detection efficiency. In this study, we fabricated a porous silicon-based DNA microarray (por-Si-microarray) using photolithographic and Si anodizing techniques. We demonstrate that DNA hybridization can be detected on a por-Si-microarray through the analysis of infrared absorption spectral profiles in the region where the vibration modes of the bases appear. This present approach demonstrates that por-Si-microarray in conjugation with infrared microspectroscopy has potential application in DNA sensing chips.",

author = "Yamaguchi, {Ryo Taro} and Miyamoto, {Ko Ichiro} and Ishibashi, {Ken Ichi} and Ayumi Hirano and Said, {Suhana Mohd} and Yasuo Kimura and Michio Niwano",

note = "Funding Information: This work was supported by a Grants-in-Aid for Basic Scientific Research (Grant Nos. 14205007 and 17206004) from the Ministry of Education, Science, Sports, and Culture of Japan. We also gratefully acknowledge Japan Science and Technology Corporation (JST) for the financial support of the Core Research for Evolution Science and Technology (CREST).",

year = "2007",

doi = "10.1063/1.2751415",

language = "English",

volume = "102",

journal = "Journal of Applied Physics",

issn = "0021-8979",

publisher = "American Institute of Physics",

number = "1",

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T1 - DNA hybridization detection by porous silicon-based DNA microarray in conjugation with infrared microspectroscopy

AU - Yamaguchi, Ryo Taro

AU - Miyamoto, Ko Ichiro

AU - Ishibashi, Ken Ichi

AU - Hirano, Ayumi

AU - Said, Suhana Mohd

AU - Kimura, Yasuo

AU - Niwano, Michio

N1 - Funding Information: This work was supported by a Grants-in-Aid for Basic Scientific Research (Grant Nos. 14205007 and 17206004) from the Ministry of Education, Science, Sports, and Culture of Japan. We also gratefully acknowledge Japan Science and Technology Corporation (JST) for the financial support of the Core Research for Evolution Science and Technology (CREST).

PY - 2007

Y1 - 2007

N2 - A method is described for the label-free detection of DNA hybridization on porous silicon (por-Si), based upon the pairing of oligonucleotide chemistry and standard silicon nanotechnology. Por-Si with a pore diameter of approximately 30 nm was used to immobilize probe DNA. Infrared microspectroscopy was employed to monitor the hybridization of probe-DNA immobilized on pore surfaces with its complementary DNA (target-DNA). The immobilization of probe DNA on por-Si facilitates hybridization detection for a small sensing area (approximately 50×50 μ m2) with a high detection efficiency. In this study, we fabricated a porous silicon-based DNA microarray (por-Si-microarray) using photolithographic and Si anodizing techniques. We demonstrate that DNA hybridization can be detected on a por-Si-microarray through the analysis of infrared absorption spectral profiles in the region where the vibration modes of the bases appear. This present approach demonstrates that por-Si-microarray in conjugation with infrared microspectroscopy has potential application in DNA sensing chips.

AB - A method is described for the label-free detection of DNA hybridization on porous silicon (por-Si), based upon the pairing of oligonucleotide chemistry and standard silicon nanotechnology. Por-Si with a pore diameter of approximately 30 nm was used to immobilize probe DNA. Infrared microspectroscopy was employed to monitor the hybridization of probe-DNA immobilized on pore surfaces with its complementary DNA (target-DNA). The immobilization of probe DNA on por-Si facilitates hybridization detection for a small sensing area (approximately 50×50 μ m2) with a high detection efficiency. In this study, we fabricated a porous silicon-based DNA microarray (por-Si-microarray) using photolithographic and Si anodizing techniques. We demonstrate that DNA hybridization can be detected on a por-Si-microarray through the analysis of infrared absorption spectral profiles in the region where the vibration modes of the bases appear. This present approach demonstrates that por-Si-microarray in conjugation with infrared microspectroscopy has potential application in DNA sensing chips.

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DNA hybridization detection by porous silicon-based DNA microarray in conjugation with infrared microspectroscopy

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