Dna ploidy of pheochromocytoma on cytology specimen by image analysis

Noriko Kimura; Mika Watanabe; Tsuneo Ookuma; Wakako Miura; Takao Noshiro; Yukio Miura; Hiroshi Nagura

doi:10.1007/BF02921474

Dna ploidy of pheochromocytoma on cytology specimen by image analysis

Noriko Kimura, Mika Watanabe, Tsuneo Ookuma, Wakako Miura, Takao Noshiro, Yukio Miura, Hiroshi Nagura

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Abstract

Pheochromocytoma usually shows prominent nuclear atypia, but the presence of such atypical cells is known to be an unreliable predictor of malignancy. DNA ploidy of pheochromocytomas has been analyzed by flow cytometry or photospectrometry on paraffinem-bedded tissue, but the results were controversial. We performed DNA analysis on cytology specimens of 11 pheochromocytomas using an image analysis system. All tumors had a mixed pattern of a large population of diploid cells and a small population of polyploid cells. DNA content correlated with nuclear size, and larger cells had more DNA content. Such larger tumor cells had polyploid nuclei, such as 4 C, 8 C, 16 C, and 32 C, in both malignant and benign pheochromocytomas. The larger polyploid nuclei may result from difficulty of duplication at the mitotic phase of the cell cycle.

Original language	English
Pages (from-to)	178-182
Number of pages	5
Journal	Endocrine Pathology
Volume	5
Issue number	3
DOIs	https://doi.org/10.1007/BF02921474
Publication status	Published - 1994 Sept 1

ASJC Scopus subject areas

Pathology and Forensic Medicine
Endocrinology, Diabetes and Metabolism
Endocrinology

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10.1007/BF02921474

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title = "Dna ploidy of pheochromocytoma on cytology specimen by image analysis",

abstract = "Pheochromocytoma usually shows prominent nuclear atypia, but the presence of such atypical cells is known to be an unreliable predictor of malignancy. DNA ploidy of pheochromocytomas has been analyzed by flow cytometry or photospectrometry on paraffinem-bedded tissue, but the results were controversial. We performed DNA analysis on cytology specimens of 11 pheochromocytomas using an image analysis system. All tumors had a mixed pattern of a large population of diploid cells and a small population of polyploid cells. DNA content correlated with nuclear size, and larger cells had more DNA content. Such larger tumor cells had polyploid nuclei, such as 4 C, 8 C, 16 C, and 32 C, in both malignant and benign pheochromocytomas. The larger polyploid nuclei may result from difficulty of duplication at the mitotic phase of the cell cycle.",

author = "Noriko Kimura and Mika Watanabe and Tsuneo Ookuma and Wakako Miura and Takao Noshiro and Yukio Miura and Hiroshi Nagura",

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T1 - Dna ploidy of pheochromocytoma on cytology specimen by image analysis

AU - Kimura, Noriko

AU - Watanabe, Mika

AU - Ookuma, Tsuneo

AU - Miura, Wakako

AU - Noshiro, Takao

AU - Miura, Yukio

AU - Nagura, Hiroshi

PY - 1994/9/1

Y1 - 1994/9/1

N2 - Pheochromocytoma usually shows prominent nuclear atypia, but the presence of such atypical cells is known to be an unreliable predictor of malignancy. DNA ploidy of pheochromocytomas has been analyzed by flow cytometry or photospectrometry on paraffinem-bedded tissue, but the results were controversial. We performed DNA analysis on cytology specimens of 11 pheochromocytomas using an image analysis system. All tumors had a mixed pattern of a large population of diploid cells and a small population of polyploid cells. DNA content correlated with nuclear size, and larger cells had more DNA content. Such larger tumor cells had polyploid nuclei, such as 4 C, 8 C, 16 C, and 32 C, in both malignant and benign pheochromocytomas. The larger polyploid nuclei may result from difficulty of duplication at the mitotic phase of the cell cycle.

AB - Pheochromocytoma usually shows prominent nuclear atypia, but the presence of such atypical cells is known to be an unreliable predictor of malignancy. DNA ploidy of pheochromocytomas has been analyzed by flow cytometry or photospectrometry on paraffinem-bedded tissue, but the results were controversial. We performed DNA analysis on cytology specimens of 11 pheochromocytomas using an image analysis system. All tumors had a mixed pattern of a large population of diploid cells and a small population of polyploid cells. DNA content correlated with nuclear size, and larger cells had more DNA content. Such larger tumor cells had polyploid nuclei, such as 4 C, 8 C, 16 C, and 32 C, in both malignant and benign pheochromocytomas. The larger polyploid nuclei may result from difficulty of duplication at the mitotic phase of the cell cycle.

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Dna ploidy of pheochromocytoma on cytology specimen by image analysis

Abstract

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UN SDGs

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