TY - JOUR
T1 - DNA polymerase λ protects mouse fibroblasts against oxidative DNA damage and is recruited to sites of DNA damage/repair
AU - Braithwaite, Elena K.
AU - Kedar, Padmini S.
AU - Lan, Li
AU - Polosina, Yaroslava Y.
AU - Asagoshi, Kenjiro
AU - Poltoratsky, Vladimir P.
AU - Horton, Julie K.
AU - Miller, Holly
AU - Teebor, George W.
AU - Yasui, Akira
AU - Wilson, Samuel H.
PY - 2005/9/9
Y1 - 2005/9/9
N2 - DNA polymerase λ (pol λ) is a member of the X family of DNA polymerases that has been implicated in both base excision repair and non-homologous end joining through in vitro studies. However, to date, no phenotype has been associated with cells deficient in this DNA polymerase. Here we show that pol λ null mouse fibroblasts are hypersensitive to oxidative DNA damaging agents, suggesting a role of pol λ in protection of cells against the cytotoxic effects of oxidized DNA. Additionally, pol λ co-immunoprecipitates with an oxidized base DNA glycosylase, single-strand-selective monofunctional uracil-DNA glycosylase (SMUG1), and localizes to oxidative DNA lesions in situ. From these data, we conclude that pol λ protects cells against oxidative stress and suggest that it participates in oxidative DNA damage base excision repair.
AB - DNA polymerase λ (pol λ) is a member of the X family of DNA polymerases that has been implicated in both base excision repair and non-homologous end joining through in vitro studies. However, to date, no phenotype has been associated with cells deficient in this DNA polymerase. Here we show that pol λ null mouse fibroblasts are hypersensitive to oxidative DNA damaging agents, suggesting a role of pol λ in protection of cells against the cytotoxic effects of oxidized DNA. Additionally, pol λ co-immunoprecipitates with an oxidized base DNA glycosylase, single-strand-selective monofunctional uracil-DNA glycosylase (SMUG1), and localizes to oxidative DNA lesions in situ. From these data, we conclude that pol λ protects cells against oxidative stress and suggest that it participates in oxidative DNA damage base excision repair.
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U2 - 10.1074/jbc.C500256200
DO - 10.1074/jbc.C500256200
M3 - Article
C2 - 16002405
AN - SCOPUS:24744472268
SN - 0021-9258
VL - 280
SP - 31641
EP - 31647
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 36
ER -